电化学发光法测定有机磷农药残留研究【毕业设计】.doc

- PAGE I - （20 届） 本科毕业设计 电化学发光法测定有机磷农药残留研究 Detection Base on Electrochemiluminescence Study of Organophophorus Pesticides 所在学院 专业班级 化学 学生姓名 学号 指导教师 职称 完成日期 年 月 电化学发光法测定有机磷农药残留研究 PAGE IV PAGE 7 摘　要 【摘要】本文研究电化学发光(ECL)的化学传感器，基于纳米二氧化锆对有机磷农药有强的吸附作用，ZrO2修饰的电极对甲基对硫磷直接检测。纳米二氧化锆修饰在玻碳电极表面（ZrO2/GCE）提供巨大的吸附表面积。修饰好的（ZrO2/GCE）电极在鲁米诺/氯化钾溶液中获取ECL背景信号，然后在一定浓度的甲基对硫磷溶液中进行吸附后获得增强的ECL信号。优化条件包括pH值、吸附时间、鲁米诺溶液浓度和光电倍增管（PMT）的电压等，甲基对硫磷的浓度检测线性范围为3.8 × 10-11~ 3.8 × 10-6 mol?L-1，检测限为1.27 × 10-11 mol?L-1 (S/N=3)。这项工作将为有机磷农药残留提供快速、简便和高灵敏度的检测方法。 【关键词】化学传感器；纳米二氧化锆；甲基对硫磷；鲁米诺 Detection Base on Electrochemiluminescence Study of Organophophorus Pesticides Abstract 【Abstract】In this paper, electrochemiluminescence(ECL)chemical sensor was modified the electrode by zirconia nanparticles, which base on Nano-zirconia have a strong absorption methyl parathion, and the modified electrode can use for the measurement of methyl parathion directly. Zirconia nanoparticles was modified on the glassy carbon electrode(ZrO2/GCE)surface, which provides a huge absorption surface area. The modified electrode (ZrO2/GCE) gain the ECL the background signal in the luminol/KCl solution,then a certain concentration methyl parathion was absorbed to the ZrO2/GC electrode surface and obtained an greatly enhanced ECL signal in the same solution. Operational parameters including pH value of the reaction medium concentration of luminol, absorption time and the potential of PMT have been optimized. he methyl parathion was determined in the range of 3.8 × 10-11 to 3.8 × 10-6 mol?L-1, with a low detection limit of 1.27 × 10-11 mol?L-1 (S/N=3).this work will provide a rapid, simple and highly sensitive method of organophophorus pesticide residues detection. 【Keywords】 chemical sensor；zirconia nanparticles；methyl parathion；luminol。 目　录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc291106536 摘　要 PAGEREF _Toc291106536