骨肉瘤肿瘤血管生成与微血管密度在瘤体内的分布差异分析.pdfVIP

ABSTRACT difference andmicrovessel thedistributionofVEGF density(MVD) Objective：Tostudy withinhumanosteosarcomaaswellaswithin ofhuman specimens xenografls insubcutisofnude the of osteosarcomacelllineMG63 mice；Toanalyzerelationship theVEGF andMVDindifferent ofthe expression region tumor；TOprovide referencetothedefinitionofsite aboutwherethe experimental clinically specimen shouldbe totheclinicalandbasicresearchaboutthe of drawn，and angiogenesis osteosarcoma． Methods：1Threeintactosteosarcomawereselectedtoestablish specimens inwhich centerweretakenasthe three．dimensionalcoordinate tumor original system as unitonthevertical 1cmwastakenbasic axis，thesagittalaxis，the point，and coronalaxistoobtain coordinates．Tissuemassesweredivided space respectively to wereimbeddedin afterfixationin10％ coordinates，and paraffin accordingspace wereusedtomaketissue onwhicheach formalin．Thentissuemasses chips point a for0．5mm．Eachtumor atissue diameter1．0ramwasordered partition produced Cut onthe atissuemass．Paraffinsectionwasfor represented chip．Eachpoint chips of