hVEGF-165基因修饰的MSCs修复犬股骨头坏死与其转归.pdfVIP

hVEGF-165基因转染骨髓间充质干细胞移植修复犬股骨头坏死及移植后的转归 第2周时骨钙素阳性细胞与GFP阳性共染的比例是850／牡4％：第8周是是 860／社6％；第12周时为90％t5％。所有标本中没有发现Ⅷ因子相关抗原的免疫 荧光与GFP共染。 结论①股骨颈截骨后固定的方法可以稳定的制作犬股骨头坏死的动物模型。② 与未转基因的间充质干细胞相比，转染hVEGF．165基因后的间充质干细胞移植 能够增加股骨头坏死区血管的数量，修复股骨头坏死的速度也更快。⑧植入股 骨头坏死区的间充质干细胞通过增殖并分化为成骨细胞而促进股骨头坏死的修 复。 关键词股骨头坏死；问充质干细胞；干细胞治疗，干细胞转归 Abstract The ofhVEGF·-1 stem mesenchymal Objectiveefficacy 65--gene--modified for of osteonecrosis evaluatedthe cells(MSCs)was repairsurgically·induced ofthefemoralin andthesurvivalanddifferentiationstatus head mongreldogs to the ofMSCs wereassessedelucidateexactmechanisminvolved implanted MSCs． inthe induced repairprocess by MethodsAnimalmodelwas inducedneck and surgically by osteetomy dividedinto mongrel hips)were three repinning．Twenty-sevendogs(54 hVEGF··165andGFP co-transfectedMSCs- equal--sizedgroups：a genes anuntrealed implantedgroup。aMSCs—implantedgmup，and group． to into stem methodwasusedintroduce cells． Lipofectin genesmesenchymal Gene-transfectedandnon4ransfectedMSCswere intothesame implanted animal’Sleflor femoralhead and andblood randomIy．Bone right seperately