微波法萃取银杏叶黄酮类有效成分.pdfVIP

微波法萃取银杏叶黄酮类有效成分 严虹霞 ( 山西省肿瘤医院药剂科，山西太原，030013) 摘要：目的：萃取银杏叶中的银杏黄酮。方法：采用单因素法，考察了粒径、辐 射功率、辐射时间、溶剂种类、固液比及萃取次数等对萃取银杏黄酮的影响，对 高效液相色谱法和分光光度法测定的主要有效成分总黄酮及山奈酚、槲皮素萃取 率作为综合指标进行评价。结果：在本实验室的条件下微波法萃取银杏黄酮的最 佳条件为：粒径0.177 mm，辐射功率700W，时间300 S ，溶剂为60%乙醇，料液 比为1：25 ，萃取3 次。在此条件下，山奈酚、槲皮素及总黄酮萃取率分别为0.273%、 0.319%和2.16%。结论：微波法适宜于萃取银杏黄酮。 关键词：微波法；银杏；黄酮 Microwave Aid Extraction of Flavonoids from Ginkgo biloba Leaves YAN Hongxia （Shanxi Provincial Tumor Hospital, Taiyuan 030013, China ） ABSTRACT：AIM: To obtain the fine flavonoids from Ginkgo biloba leaves with microwave radiation heating. METHODS: The effects of particle size, microwave radiation power, microwave radiation time, solution type, solid-liquid ratio and extraction times on extraction were studied. The flavonoids contents was analysis by UV spectrophotometer, Keampferol and Quercetin was test by HPLC. RESULTS: the optimal conditions were determined: average particle size of 0.177 mm, microwave radiation power of 700 W, microwave radiation time of 300 S, solution type of 60% ethanol, solid - liquid ratio of 1:25 and extraction times of 3. In this conditions, the extraction ratio of Keampferol, Quercetin and total flavonoids were 0.273% ，0.319% and 2.16 ％. CONCLUSION: Microwave aid extraction is a good method for extraction of flavonoids from Ginkgo biloba leaves. KEYWORD: Microwave aid extraction; Ginkgo biloba; Flavonoids 严虹霞，主管药师，硕士。研究方向：中药萃取及药代动力学研究，临床药学。电话：0351-4651580 。E-mail ： hongxia.yan@ 1 中图分类号Q58 文献标识码A 文章编号 银杏叶中含有多种生理活性物质，如黄酮类化合物、萜内酯等，具有改善心 脑血管循环、抗癌、抗衰老及降低胆固醇等作用，以银杏叶黄酮类化合物萃取物 [1] 制成生物医药深受生物制药研究的重视 。目前银杏常用的萃取方法有水提法、