含小檗碱类中药品质生物评价与控制的初步研究-药物化学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 4 4 bio-thermokinetics method as objective and effective means. The biopotency of Cortex Phellodendri, Cortex Phellodendri Amurene, Coptis Chinensis and Radix Berberidis under the limits were 70 U/g, 15 U/g, 440 U/g and 30 U/g tentatively, and the confidence rate should not exceed 10 percent. The relevance of the test results has been analyzed comprehensively, conducted the chemical characteristics-biopotency information, biopotency - biopotency information of the relevance analysis showed that: between the chemical characteristics and biopotency information showed significantly positive correlation (the correlation coefficient r=0.8517, P0.01); The biopotency information of cup-plate method and bio-thermokinetics method showed the good correlation and consistency (the correlation coefficient r=0.9861, P0.01), It suggested that the two biopotency measurement methods can be used to supplement and consummate the quality control and evaluation for TCD which contained berberine. Compared with the relevance of Chemical analysis and biological evaluation methods, the relevance of different biological evaluation methods, the results showed that biological evaluation method can be used in TCD which contained berberine quality evaluation; The cup-plate method and bio-thermokinetics method both can be used for TCD antimicrobial potency measurement. The systems of TCD which contained berberine quality evaluation and control were established preliminarily. So was the quality evaluation and control for TCD which contained berberine according to the common requirement of pharmacopoeia, analysis of multiple key components, and detection of biopotency. And the biopotency measurement method is the core of the quality control and evaluation for TCD. Conclusion: According to above-mentioned results, it could be concluded that this new pattern and method demonstrated its contribution and valued for the quality control for TCD which contained berberine prelim