固相微萃取-色谱联用在农药类环境激素检测中的应用研究-环境科学专业论文.docxVIP

II II 摘 要 环境激素是存在于环境中的类激素物质，它们与存在于人体内的激素具有相近 的分子结构，进入人体后，就会与相关受体结合，引起相应的生物学反应，使组织 和器官发生不同程度的病变。环境激素的风险评价已经成为国际上环境科学领域中 的热门研究课题。本论文以农药类环境激素中的有机磷农药和氨基甲酸酯类农药为 研究对象，根据有关组织或法规对农药类环境激素的限量要求及其性质，选用合适 的检测仪器，建立分析方法和线性范围。 论文对环境激素的分类、危害以及国内外的研究现状进行了综述，并介绍了环 境激素检测中的样品前处理技术和所使用的分析仪器，详细阐述了固相微萃取技术； 论文通过对蔬菜样品的前处理技术讨论，以及萃取条件和气相色谱条件的优化，建 立了固相微萃取-气相色谱联用检测蔬菜中残留有机磷农药的方法；论文通过改进固 相微萃取装置与高效液相色谱的联用，以及固相微萃取的萃取条件和高效液相色谱 条件的优化，建立了固相微萃取-高效液相色谱联用测定蔬菜中残留氨基甲酸酯类农 药的方法。 关键词：固相微萃取；气相色谱法;有机磷类农药；高效液相色谱法；氨基甲酸 酯类农药 III III Abstract Environmental hormone is hormone-like materials present in the environment. They have similar molecular structure with the hormones that exist in the human body. When they enter the human body, they will be associated with related receptor and cause the corresponding biological response, lead to different degree lesions of tissues and organs. Evaluation of the risk of environmental hormone has become popular research topic in the field of environmental science throughout the world. Organic phosphorus pesticides and carbonate pesticides in pesticides environmental hormone is studied in this paper. According to the relevant laws and regulations on the limitation requirement and nature of pesticides environmental hormone, the paper selects suitable detecting instruments and establishes analytical methods and the linear range. By optimizing extraction conditions and chromatography conditions, the paper sets up a method that tests organic phosphorus pesticides retained in vegetables by SPME coupled to GC and a method that tests carbonate pesticides retained in vegetables by SPME coupled to HPLC. And this paper provides a new way of green analysis for the detection of pesticides environmental hormones retained in vegetables and it is of better practical application value；A literature review is first given on the classification, disadvantage and recent research going-on of environmental hormone, with an introduction of sample pretreatment technique and the analysis instrument, both o