广西黑鸡禽白血病的净化与病毒株全基因组序列分析-预防兽医学专业论文.docxVIP

II II 在 ALV 病毒的感染，并从黑鸡中成功分离并鉴定出 2 株 ALV-J，分别命名 为 GXD2 和 GXH3；同源性分析结果显示，GXD2 和 GXH3 两毒株 gp85 和 gp37 的氨基酸同源性高达 99%以上，与蛋鸡参考株 SD07LK1 的遗传距离 较近；与参考株的同源性分析结果显示，全基因组的同源性在 93.7%以上； gag 和 pol 基因相当保守，同源性在 94%以上；UTR 区的变异较大，3＇UTR 的变异比 5＇UTR 大；两毒株都有完整的 E 元件。 研究结果表明：广西黑鸡前期的净化取得了良好的效果，净化方法可 行。同时，成功从黑鸡中分离到了两株 ALV-J，且首次完成了广西 ALV-J 分离株核酸全基因组序列的测定和分析。 关键词：禽白血病 净化 广西黑鸡 病毒分离 ALV-J 全基因组 序列 分析 III III AVIAN LEUKOSIS ERADICATION AND WHOLE GENOME SEQUENCES ANALYSIS OF AVIAN LEUKOSIS VIRUS STRAINS ISOLATED FROM GUANGXI BLACK CHICKEN ABSTRACT Avian leukosis (AL) is an immunosuppressive tumor disease caused by avian leukosis virus (ALV). The main natural host of ALV is chicken. Although its morbidity and mortality rates are low, the productivity of chicken flocks will decline when it is infected with ALV, and then cause huge economic losses in aviculture. At present, the generally accepted effective methods of prevention and control of AL is to eradicate ALV from the Flocks. In Guangxi province, black chicken is one of characteristic local breeding with large scale. We selected two poultry-farms DGGX4 and DGGX11 in Guangxi to carry out the eradication of AL and related research. The first aim was to eradicate AL out of from black breeding chicken in two poultry-farms DGGX4 and DGGX11. Applying commercialization ELISA detection kits of ALV p27 antigen, A/B and J antibody to detect samples of anus PAGE PAGE VI swab/plasma (cock), egg white (hen) and serum collected from the poultry-farms. The chicken which antigen and antibody were positive will be eliminated. After eradication of the third generation, in DGGX4 poultry-farm, the p27 antigen positive rate of black breeding chicken was from 12.4% down to 0.52%, subgroup A/B and J antibody positive rates were 0%, eradication effect was outstanding. After eradication of the fourth generation, In DGGX11 poultry-farm, the p27 antigen positive rate of black breeding chicken was from 7.71% down to 2.92%, in the third generation of eradication,