利用荧光原位杂交技术分析新合成异源四倍体拟南芥-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2010, 36(7): 1216−1220 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01216 利用荧光原位杂交技术分析新合成异源四倍体拟南芥 位 芳 张改生* / / , 712100 : , , , , A. suecica , DAPI ; , : ; ; ; DAPI; FISH Analysis of Resynthesized Allotetraploid Arabidopsis * WEI Fang and ZHANG Gai-Sheng Key Laboratory of Crop Heterosis of Shaanxi Province, Wheat Breeding Engineering Research Center, Ministry of Education, Northwest AF Uni- versity, Yangling 712100, China Abstract: Allopolyploidy breeding or introduction of preferential gene(s) via allopolyploidization is widely attempted in produc- tion of new crops. Thus at the onset of establishment of new allopolyploids, the functional gametes are reproduced conditioning that normal events such as synapsis and pairing and correct segregation of homologous chromosomes are guaranteed with the avoidance of possible interference of homoeologous chromosomes during meiosis. In the present study, we reported on meiotic synapsis and pairing of homologous chromosomes during the development of pollen mother cells in the newly resynthesized al- lotetraploid Arabidopsis aided with DAPI staining and FISH technique. The results showed that the correct number of nuclear chromosomes and