医学-北华大学学报.DOC

PAGE PAGE 4 (二号黑体)磁性Fe3O4纳米颗粒在家兔体内的分布及其药代动力学 (小四号楷体) 孙维琦1，刘 通2，张 晶1，李 环1 (小五号宋体)（1.北华大学公共卫生学院，吉林 吉林132013；2.吉林化学工业集团公司总医院，吉林 吉林132021） (小五号黑体)摘要：(小五号黑体)目的(小五号宋体)研究磁性Fe3O4纳米颗粒在家兔体内组织超微结构的分布、血药浓度-时间曲线及对血液生化指标的影响。(小五号黑体) 方法(小五号宋体)家兔耳缘静脉注射磁性Fe3O4纳米颗粒，于不同的时间点耳缘静脉采血，测定血药浓度，计算药代动力学参数。另将24只家兔按体重随机分成对照组、低剂量组、中剂量组、高剂量组，耳缘静脉注射不同剂量磁性Fe3O4纳米颗粒24h后取脏器组织及血液， 测量磁性Fe3O4纳米颗粒在家兔体内各脏器的分布，电镜观察肝及脾超微结构改变及纳米颗粒分布，对家兔血清生化指标变化进行测定。(小五号黑体)结果(小五号宋体)纳米Fe3O4在家兔的体内随着时间的增加其含量逐渐下降，符合药代动力学二室曲线模型；纳米Fe3O4在家兔体内分布具有特异性，主要分布在脾脏和肝脏中的吞噬细胞内，且在同一器官不同剂量组间分布也有差异；血清生化指标测定表明家兔血清中碱性磷酸酶（ALP）、门冬氨酸氨基转移酶（AST）、尿酸（URIC）含量有所改变。 (小五号黑体)结论(小五号宋体) 磁性Fe3O4纳米颗粒在家兔体内主要分布在脾脏和肝脏，在组织超微结构下主要分布在吞噬细胞内，血液生化指标没有大的改变。 (小五号黑体)关键词：(小五号宋体) 磁性Fe3O4纳米颗粒 ; 药代动力学 ; 组织超微结构分布 ; 血液生化指标 (小五号黑体)中图分类号：(小五号宋体) R994.6 (小五号黑体)文献标志码：(小五号宋体)A (三号黑体) Tissue Distribution and Pharmacokinetics of Magnetic Nano-Fe3O4 (小五号Times New Roman体)Sun WeiQi1 ，Liu Tong2 ，Zhang Jing1，Li Huan1 (小五号Times New Roman斜体)(1.School of Public Health，Beihua University，Jilin 132013，China; 2.General Hospital of CNPC in Jilin，Jilin 132021，China) (小五号Times New Roman 加黑)Abstract: Objective (小五号Times New Roman体)To study tissue distribution, concentration-time curve and effects on serum biochemical parameters of magnetic nano-Fe3O4 in rabbits. (小五号Times New Roman 加黑)Method Magnetic nano- Fe3O4 Was injected by ear vein of rabbit. The blood was got on different time after intravenous injection. Concentration of Nano- Fe3O4 in rabbit blood was tested by ICP-OES and pharmacokinetic parameter was calculated. Another 24 rabbits were evenly randomized into four groups according to body weight：normal control group, low-dose group, middle-dose group, high-dose group. Magnetic nano- Fe3O4 of different dose were injected by ear vein. The blood and different organs were got after intravenous injection 24 hours. The distribution of Nano- Fe3O4 in different organs were tested and ultramicrostructure liver and spleen tissues were observed by TEM , biochemical parameters of serum in rabb