棕榈酰化蛋白及蛋白质的棕榈酰化研究进展-亚热带植物科学.PDF

2018,47(4): 395 ～403. Subtropical Plant Science 棕榈酰化蛋白及蛋白质的棕榈酰化研究进展 丁玉娇，韩颖颖，周婧雯 (上海理工大学医疗器械与食品学院，上海 200082) 摘 要：蛋白质 S-棕榈酰化是最常见的具有 16 碳脂肪酸棕榈酸酯的脂质修饰形式，调节蛋白质的运输和功能。 文中主要概括从植物到哺乳动物中发现的具有棕榈酰基转移酶活性的保守 DHHC 蛋白家族，并介绍蛋白质棕 榈酰化的研究方法，及检测棕榈酰化蛋白质的位点预测方法(CSS-Palm 、NBA-Palm 、TermiNator2) 、放射性标 记法(用 3H 棕榈酸酯或 125I-IC16 棕榈酸酯)和非放射性标记法(化学标记和质谱法) ，总结蛋白棕榈酰化的抑制技 术以及抑制剂类型( 包括2-溴棕榈酸酯、浅蓝菌素和衣霉素) 。同时概括蛋白棕榈酰化在植物胁迫中的响应，展 望其在植物抗逆中的应用前景。 关键词：棕榈酰化修饰；DHHC 蛋白；功能；研究方法；抑制剂 Doi: 10.3969/j.issn.1009-7791.2018.04.017 中图分类号：Q51 文献标识码：A 文章编号：1009-7791(2018)04-0395-09 Progress in Research of Palmitoylated Proteins and Protein Palmitoylation DING Yu-jiao, HAN Ying-ying, ZHOU Jing-wen (School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200082, China) Abstract: Protein S-palmitoylation, the most common lipid modification with the 16-carbon fatty acid palmitate, provided an important mechanism for regulating protein trafficking and function. This article summarized a family of conserved DHHC proteins with palmitoyltransferase activity that had been discovered from plants to mammals. The method of protein palmitoylation was so concluded. The paper also introduced the research methods of protein palmitoylation, the prediction methods of palmitoylation sites (CSS-Palm, NBA-Palm, TermiNator2), radioactive labeling (radiolabeling with 3 125 H palmitate or I-IC16 palmitate) and non-radioactive labeling (chemical labeling and mass spectrometry) to detect palmitoylated proteins. Next, techniques to inhibit protein palmitoylation were described. These i