MHC分子与移植免疫.pptVIP

2、细胞学分型技术-混合淋巴细胞反应（MLR）法 两种方法：双相混合淋巴细胞培养和单相混合淋巴细胞培养。 双相混合淋巴细胞培养： 受者的淋巴细胞+供者淋巴细胞 细胞增殖 增殖程度与两个个体组织相容性不配合的程度成正比，即为双相混合淋巴细胞培养。 单相混合淋巴细胞培养： 如果一方是先用丝裂霉素Ｃ或 致死量射线处理，使其失去应答力，但仍保持刺激能力，把未处理的淋巴细胞和经上述处理的淋巴细胞一起培养的方法即为单相混合淋巴细胞培养 Mixed lymphocyte reaction to determine identity of class II HLA antigens between a potential donor and recipient. Lymphocytes from the donor are irradiated or treated with mitomycin C . If the class II antigens on the two cell populations are different, the recipient cells will divide rapidly and take up large quantities of radioactive nucleotides into the newly synth