浮萍转录组数据SSR位点的生物信息学分析-中国科学院成都生物研究.PDF

2015-06-25 DOI: 10.3724/SP.J.1145.2014.04027 / Chin J Appl Environ Biol 2015 21 ( 3 ) : 401-405 SSR* 1, 2 1 1 1 1 1** Trinity 74 797 unigene MISA unigene SSR 14 250 SSR 12 707 unigene 19.05% SSR 19 bp 1/6.57 kb SSRsSSRs70.74%25.37% SSR 185 AG/CT CCG/CGG SSRs 69.19% 8.84% SSRs 3 2 35 SSR CLC Q811.4 : Q949.717.303 BBioinformatic analysis on SSR information in duckweed transcriptomeioinformatic analysis on SSR information in duckweed transcriptome * 1, 2 1 1 1 1 1** SUN Jiaolong , FANG Yang , JIN Yanling , MA Xinrong , TAO Xiang ZHAO Hai 1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China AAbstractbstract Duckweed is the smallest flowering plants in the world, growing faster than any other flowering plants. This research assembled with Trinity the raw data obtained by high-throughput RNA sequencing and got 74 797 unigenes. Overall, 14 250 SSRs distributed in 12 707 unigenes were detected, accounting for 19.05% of all unigenes. The average length of the transcriptomic SSRs was 19 bp, and the distribution distance 6.57 kb. The dinucleotide repeats and the trinucleotide were the main types, accounting for 70.74% and 25.37%, respectively, of all the SSRs.