光镊技术在液相体系微区特性参数测量中的应用-光学专业论文.docxVIP

中国科学技术大学硕士学位论文 中国科学技术大学硕士学位论文 摘要 技术上的研究成果提出的。我们从物理和技术的角度讨论了相关检测分析方法 和技术，提出了配套的检测装置的设计方案，完成了原理性装置的搭建和验证 工作，以及样机的设计、研制及测试工作。结果显示，样机已具备观测和处理 生物样品的荧光图像等最基本的功能。论文还给出了进～步完善仪器性能的改 进意见。以上工作进展，为达成最终的研制目标，成就造福人类的美好愿望， 奠定了工作基础。 关键词：光镊，温度，黏度，布朗运动，生物传感器，检测仪 中国科学技术大学硕士学位论文 中国科学技术大学硕士学位论文 摘要 Abstract Along with the progressing of science and technology，the understanding of the matter characteristics is spreading to micro scale．So the measurement of the matter properties in a wanted micro-area becomes much more important than before．One of the main works in this thesis is to develop a new method based on optical tweezers for studying the physical characters in micro—area，especially the temperature．The temperature is not only all important physical quantity,but also one of the seven fundamental quantities in the International System of Units．The physical and chemical characteristics of all objects in nature are temperature dependent．When the micro scale matter is studied such as in the case of optical tweezers application，in order to understand better the behavior of the trapped obj ect，it is necessary to know the local temperature field with high space resolution，that is，the measurement in micro area is needed． In this work new method of measuring the temperature at wanted micro-area in liquid by using the technology of optical tweezers is developed．All methods of measuring the temperature based the relationship between the temperature and some property of the matter．The method here is directly based the relationship between the temperature and the Brownian motion of a particle suspending in the liquid．Here the Brownian particle serves as the temperature sensor，of which the size is usually around micron，much smaller than the size of the ordinary sensor．This fact enables the temperature measurement with high space resolution．At the same time，the feature of the opti