野生大豆碳酸盐胁迫应答基因GsMIPS2的克隆及功能分析-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2015, 41(9): 13431352 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.01343 野生大豆碳酸盐胁迫应答基因 GsMIPS2 的克隆及功能分析 陈 晨1 孙晓丽2 刘艾林1 端木慧子1 于 洋1 肖佳雷1 朱延明1,* 1 , 150030; 2 , 163319 : (Glycine soja L. G07256), , , -1- , cDNA, GsMIPS2 PCR , GsMIPS2 , T-DNA atmips2 , , GsMIPS2 , atmips2 , GsMIPS2 : ; GsMIPS2 ; -1- ; Cloning and Functional Analysis of Glycine soja Bicarbonate Stress Responsive Gene GsMIPS2 1 2 1 1 1 1 CHEN Chen , SUN Xiao-Li , LIU Ai-Lin , DUAN-MU Hui-Zi , YU Yang , XIAO Jia-Lei , and ZHU Yan-Ming 1,* 1 College of Life Sciences, Northeast Agricultural University, Harbin 150030, China; 2 College of Agronomy, Heilongjiang Bayi Agricultural Univer- sity, Daqing 163319, China Abstract: To identify the key functional genes in response to bicarbonate stress in Glycine soja L. G07256, we constructed a gene expression profile under bicarbonate treatment using high throughput RNA-seq data, from which we selected a myo-inositol- 1-phosphate synthase gene whose expression was significantly induced by bicarbonate stress. By homology-based cloning, we acquired its full length cDNA and termed as GsMIPS2. The results of quantitative real-time PCR demonstrated the bicarbonate stress induced expression of GsMIPS2 and its tissue expression specificity. We also verified the func