膜蛋白交联对红细胞膜变形性与流动性影响.PDFVIP

19 2 Vol. 19No.2 2000 6 Beijing Biomedical Engineering Jun. 2000 膜蛋白交联对红细胞膜变形性及流动性的影响 * 孙大公　施　岩 　陈　凯　谢利德　姚伟娟　文宗曜 (100083) * (067000) , E Lm D H MSL ( ESR) , , , ( ) ( ) , E Lm 0 , , ( HS) , ( NH) [ 1] Manno , I beta-spectrin , beta-spec- trin ; [ 2] An XL T akakuwa 4. 1 3 , 4. 13 , 3 , [ 3] , Gwozdzinski , , , [ 4] , , [ 5] [ 6] [ 7] , [ 8] , D H , MSL (ESR) , [ 7, 9, 10] E Lm 1 1. 1 , ; ( Glutaraldehyde) ; : ( 1966) , , 2 : 97 ( MSL Sigma, ) ; D H ( 1, 6-1, 3, 5-, Sigma ) ; V (polyvinlpyrolidone, 30kD) Bruker ES 300 ; ( HIT ACHI) 850 ; GYJ- I , ; LG10-3A 1. 2 1. 2. 1 (resealed ghost) , , , BS , , 2 , 7. 9 4 401 , pH , 12000rpm , , 4 , pH7.4 BS 1. 2. 2 (ESR) ( MSL) 5 , 200Ll, 01/ 40001/ 30001/ 20001/ 1000 (V/ V) BS 5min, , BS , 15Ll ( MSL) 800Ll BS , 4min, 4 BS 34 ( 10000rpm, 10min) 1. 2. 3 01/ 40001/ 30001/ 20001/ 1000 1/ 500 5min, 400Lg D H ( ) 1. 2.4C= 0 ( DId) , , BS 01/ 40001/ 30001/ 20001/ 1000 BS , 7 210 / ml, 5min 3% V ( 30kD, 0.04% ) , = 0 ( d) , : C DI = 200s- 1 1. 2. 5( DImax) (t0. 5) ( 1. 2.4) ( 1. 2.4)