亚硒酸钠、硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外研究.doc

亚硒酸钠、硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外研宄 摘要：［目的］观察亚硒酸钠（Na2SeO3）、 硫酸镁（MgS04）对二氧化硅（石英）致巨噬细胞 （RAW264.7）释放肿瘤坏死因子（TNF-a ）、白介素-1 （IL-1）的单独拮抗作用以及对人胚肺成纤维细胞羟脯 氨酸（Hyp）合成水平变化的单独以及联合拮抗作用。 ［方法］对数生长期的RAW264.7细胞，调整浓度为 2X 105个/ml。在染200 mg/L石英同时单独加入不同 浓度Na2SeO3、MgSO4溶液，24 h后测定培养液中TNF 和IL-1水平，并取培养液与人胚肺成纤维细胞共同培 养，48 h后测定人胚肺成纤维细胞培养液中Hyp含量。 另外，在染石英同时联合加入不同浓度的Na2SeO3和 MgSO4溶液，培养24 h后取培养液与人胚肺成纤维细 胞共同培养，48 h后测定其中的Hyp含量。［结果］ ①与石英组比，Na2SeO3对RAW264.7细胞释放TNF- a无明显影响，MgSO4可以显著降低TNF-a的活性（P 关键词：亚硒酸钠；硫酸镁；石英；巨噬细胞； 人胚肺成纤维细胞；肿瘤坏死因子；白介素-1;羟脯 氨酸 A Study about the Effect of Sodium Selenite and Magnesium Sulfate on Cytokine Releasing and the Hydroxyproline Synthetizing Induced by Quartz CHEN Guo-yin，TANWei-jun*，YIN Hai-rong, HE Zheng, SUN Yan (School of Public Health， Southeast University, Nanjing 210009，China) Abstract： [Objective] To investigate the effect of sodium selenite and magnesium sulfate which were used separately on TNF-a and IL-1 releasing from RAW264.7 macrophage treated with quartz and which were used separately and/or combined on the hydroxyproline synthetizing in human embryonic lung fibroblast also treated with quartz. [Methods] RAW264.7 and human embryonic lung fibroblast were cultured at 37°C with 5% carbon dioxide. RAW264.7 were prepared during the growth period of logarithm in the concentration of 2 X 105 /ml and poisoned with quartz (200 mg/L)，at the same time sodium selenite and magnesium sulfate in different concentration were added separately. After 24 hours，TNF-a and IL-1 in RAW264.7 culture fluid were measured .Then the RAW264.7 culture fluid and the human embryonic lung fibroblast were cultured together. After 48 hours， the hydroxyproline in human embryonic lung fibroblast culture fluid were measured. In addition, when the RAW264.7 was poisoned with quartz, sodium selenite and magnesium sulfate were added together simultaneously. Then followed the same procedure as above mentioned and the hydroxyproline in human embryonic lung fibroblast culture fluid were measured too after 48 hou