泛素及其类似物蛋白质组学研究进展谭锋维.pdfVIP

2007 　1 Janua ry 2007 27 　 1 Basic C lin ica lM edicine V ol. 27　No. 1 :1 00 1-6 32 5(2 007 )0 1-0 00 8-0 8 　 1 2 2 1 谭锋维 , 陆丽芳, 王　晖, 彭小忠 (1. , 100005;2., 201203) :, (ubiqui in-like m odif iers, Ub ls) 。, /ubls, 、、、 。, , Ub ls。 /U bls, ubls, /Ub ls。 /Ub ls , 。, /Ub ls, 。 U bls 。 :;; :R 34　　:A Pro eom ic approaches o pos-ransla iona lm odifica ion by ubiqu i in fam ily pro eins 1 2 2 1 TAN Feng-we i, LU L-i fang , WANG H ui, PENG X iao-zhong (1.Ins i u e of B as ic M edical S ciences, CAM S PUM C, Beijing 100005;2.Shanghai G enom ics, Inc. , Sh anghai 20 1203, C h ina) Abstract:Pos-ransla iona lm odifica ion by ubiqui in and ub iqui in-like m odifiers(Ub ls)is one of he m os mi po-r an m echanism s regula ing a w ide range o f cellu lar processes in eukaryo es. P reviou s research show ed ha, h rough covalen ly m od ifica ion by ubiqui in o r ubls, he subs ra e pro eins can be regula ed in m any diffe ren w ays like s a- b ili y, subce llu lar localiza ion, enzym a ic ac ivi y, pro e in-pro ein in erac ion and so on. The refo re, w e be lieve, ha ubiqui in and ub ls p lay ve ry mi po r an ro les in ce llular and bio log ica l processes by m odifying plen y o f pro eins. To be e r unders and he ub iqu i in and ubls sy s em, pro eom ic approaches have been deve loped o pu rify and iden -i fy m o re pro e in subs ra es. L arge-scale idendifica ion of ubiqui in /ubls-m odifica ion si es by m ass spec rom e ry is par icularly impor an fo r unders anding he m olecula rm echanism and func ion of ubiqui in /ub lsm od ifica ion. Up