固定化微生物捷径生物脱氮的研究-微生物学专业论文.docxVIP

广西大学硕士学位论文 广西大学硕士学位论文 固定化微生物捷径生物脱氮的研究 作联合捷径脱氮试验，27h脱氮率为97．53％；并作本校富营养化湖水 的脱氮应用试验，处理12h，NH4+-N去除率为84．06％。 关键词：捷径生物脱氮固定化微生物亚硝化细菌反硝化细菌 菌种分离筛选条件选择 广西大学硕士学位论文 广西大学硕士学位论文 同定化微生物捷径生物脱氮的研究 STUDIES oN SHoIU’．CUT BIODENITRIFICATIoN BY IMMOBILIZED MICROBE AB STRACT Biodenitrifieation is one of important techniques in environmental engineering．The short-cut biodenitrification is that NH4+-N is oxidited into N02‘and then is reduced directly into N2，the process needs nitrite bacteria and denitrification bacteria．In this paper,adopted augmenting culture in nitrite bacteria culture media and the method of silica gel plate isolation，3 1 strains of bacteria were isolated from vegetable garden soil of our sch001．The color reaction test was carried out with Griess reagent and culture fluid，which was regarded as the index to determine producing NOr or not．1 3 strains which color were deep were obtained and they were further rescreened by doing nitrite test．A strain N4(coded N4，the same to the following)with higher rate of nitrosification was picked up after rescreened；A strain Bos(coded Bos，the same to the following)with higher rate of denitriflcation was obtained after isolated and rejuvenated from our lab conserving mixed denitrifying bacteria culture．Then made them into immobilized cells respectively using polyvinyl alcohol and sodium alginate as carrier．The experiments were 广西大学硕士学位论文 广西大学硕士学位论文 固定化微生物捷径生物脱氮的研究 carried out for the selection of nitrosifieation and denitrification conditions．The results were as follows．At the temperature of 25-306C，initial pH value of 7．5-8．0，alkalinity of NaHC03 concentration 1 700mg／L，NI-h+-N concentration of 1 50mg／L，aerations of 1 1 0rpm， cultured 24h，immobilized nitrite bacteria N4’S NH4+-N removal efficiency reached 99．47％，accumulative volume of N02’N was 1 1 6．65mg／L．Under the condition of temperature 25-30 6C，initial pH value 7．0-8．0，N02。-N concentration 2