雌激素激活gper-egfr-erk 通路促进人乳腺癌skbr-3 细胞系增殖.docVIP

. . 雌激素激活GPER-EGFR-ERK通路促进人乳腺癌SKBR-3细胞系增殖 李维东，罗浩军，李振华，余腾华，杨光伦，涂 刚 （400016 重庆，重庆医科大学附属第一医院内分泌乳腺外科） [摘要] 目的 探讨G蛋白偶联雌激素受体（G protein-coupled estrogen receptor,GPER）介导雌激素对人乳癌细胞系SKBR-3增殖的影响。方法 激光共聚焦显微镜扫描检测钙离子探针标记的细胞内钙离子浓度随时间的变化，CCK-8法观察测量细胞的增殖生长，流式细胞术检测细胞周期，Western blot 检测磷酸化细胞外信号调节激酶（phospho-extracellular regulate ekinase，p-ERK）的相对表达量。 结果应和方法相对应。注意表述时的归纳总结，并突出实验组的结果；要把做过统计学分析得出主要结论的数据和统计学结果全部写入，请添加，英文也注意一致；我加入了部分结论数据，如有不妥还请给出修改意见 17-β雌二醇（E2）与GPER激动剂（G1）刺激SKBR-3细胞后，细胞内钙离子浓度从120s开始迅速升高，细胞增殖与对照组相比显著增加，CCK-8测定的相对细胞数分别是对照组的（2.08±0.07）倍和（2.0±0.04）倍，流式细胞术检测细胞S期及G2～M期即增殖指数PI（43.12%±0.38%,42.43%±0.52%）较对照组（29.19%±0.29%）增加（P〈0.05）,Western blot 显示p-ERK表达量在E2作用组显著高于对照组（P〈0.05）。GPER特异性抑制剂G15 、EGFR拮抗剂AG1478、ERK拮抗剂U0126均可抑制E2、G1触发的相应变化，PI3K拮抗剂WM则不能。结论 雌激素激活GPER-EGFR-ERK通路促进人乳腺癌SKBR-3细胞系增殖。 应和方法相对应。注意表述时的归纳总结，并突出实验组的结果；要把做过统计学分析得出主要结论的数据和统计学结果全部写入，请添加，英文也注意一致； 我加入了部分结论数据，如有不妥还请给出修改意见 [关键词] 雌激素；GPER ；SKBR-3；增殖 [中图法分类号] R737.9 [文献标志码] A Estrogen activatesGPER-EGFR-ERK pathway to promote the proliferation of human SKBR-3 breast cancer cell line Li Weidong，Luo Haojun，Li Zhenghua，Yu Tenghua，Yang Guanglun，Tu Gang（Department of Endocrine Breast Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016） [Abstract] Objective To explore the effect of G protein coupling estrogen receptor (G protein-coupled estrogen receptor, GPER) induced by estrogen on the proliferation in human SKBR-3 breast cancer cell line. Methods Calcium influx, cell growth ability and cell cycles were checked by confocal laser scanning microscopy, CCK-8 assay and flow cytometry in the presence of different drug treatment, respectively. Relative expression levels of extracellular signal regulating kinase phosphorylation (Phospho-extracellular regulated kinase, p-ERK) was detected by immune-blotting. Results After treatment of 17-β estradiol (E2) or GFER specific agonist (G1), inner cells calcium ion concentration was shifted quickly starting from 120 seco