海洋真菌Phomopsissp.产抗肿瘤药物.PDFVIP

DOI :10.16606/ki .issn0253 -4320.2008.s2.028 28(2) 现代化工 Oct.2008 2008 10 Modern C emical Industry ·275 · Phomopsis sp . 1 2 2 1 1 陈劲平,黄耀坚, 沈月毛, 敬科举, 卢英华 (1.厦门大学化学化工学院化学工程与生物工程系, 福建厦门361005; 2.厦门大学生命科学学院, 福建厦门361005) :Phomopsis sp ., (Deacety Mycoepoxydiene)。Phomopsis sp .A123 818 , 。 , 。, 、 。:PD ; ;, 25%。 :;;; :TQ464　 :A 　 :0253-4320(2008)S2-0275-03 Optimization of medium components for production of antitumor agent Deacety Mycoepoxydiene by marine endophytic fungi Phomopsis sp . 1 2 2 1 1 CHEN Jin-p ing , HUANG Yao-jian , SHEN Yue-mao , J INGKe-ju , LU Ying -hua (1.Department of C emical and Bioc emical Engineering, College of C emistry and C emical Engineering, Xiamen University, Xiamen 361005, C ina;2.Sc ool of Life Science, Xiamen University, Xiamen 361005, C ina) Abstract:Deacety Mycoepoxydiene is a novel antitumor agent extracted from a marine endop ytic fungi Phomopsis sp . A123.In order to improve t e yield of Deacety Mycoepoxydiene,t e liquid fermentation medium composition is optimized by a one-factor-at-a-time met od for a mutant No.818,w ic is derived from Phomopsis sp .A123.Potato dextrose (PD)culture medium is selected as t e preliminary culture medium, and t e optimal carbon and nitrogen sources for liquid fermentation are investigated.T e effect of several vitamins, including lipoic acid, VB , VB and biotin on t e accumulation of Deacety 12 1 Mycoepoxydiene is also studied.T e results s ow t at t e optimal carbon and nitroge