高效液相色谱法检测哌虫啶在稻田水土壤稻米和小麦粉中的残留.PDF

5 3 Vol. 5 No. 3 2014 3 Journal of Food Safety and Quality Mar. , 2014 丛路静, 刘纪松, 王美云, 王鸣华* (, 210095) 摘 要: 目的 方法 , , 结果 0.5~10 mg/L , , 0.9998, 1.0 ng 78.50%~96.61%, 2.54%~7.94% 0.05 mg/kg结论 , , , , 关键词: ; ; Determination of paichongding in paddyfield water, soil, rice and wheat by high performance liquid chromatography * CONG Lu-Jing, LIU Ji-Song, WANG Mei-Yun, WANG Ming-Hua (College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) ABSTRACT: Objectives To establish a high performance liquid chromatography (HPLC) method for determination of paichongding (IPP) in paddyfield water, soil, rice and wheat. Methods The paddyfield water was extracted with dichloromethane, while the soil, rice and wheat were extracted with acetonitrile and determined by HPLC with UV detector. Results The results showed a good linearity between the concentration of IPP and the peak areas in the range of 0.5~10 mg/L with r=0.9998. The limit of detection (LOD) was 1.0 ng. The average recoveries were 78.50% to 96.61%, and the relative standard deviations (RSD) ranged from 2.54% to 7.94%. The limit of quantification (LOQ) was 0.05 mg/kg. Conclusion The method is accurate, reliable, simple, rapid, and less organic solvent consumption, and it could meet the require