高效液相色谱-蒸发光散射检测法测定大枣提取液中的可溶性糖.DOC

高效液相色谱-蒸发光散射检测法测定大枣提取液中的可溶性糖 依明.尕哈甫 陈鑫 王耀欣 郑丽英 殷其蕾 贺锐锐 詹先王 何希辉 韩南银* (北京大学药学院 中国北京 100191) 摘 要：建立了大枣中可溶性糖的液相色谱-蒸发光散射分析方法。各组分以GRACE HYPERLINK /Prevail/10222424253.html \t _blank Prevail ES糖分析柱分离，并与蒸发光散射检测器联用，采用乙腈:水(72:28v/v)作为流动相，流速1.0 mL/min，蒸发光散射检测器漂移管温度为80 ℃，载气为空气，流速2.00 L/min。采用外标法定量，鼠李糖、阿拉伯糖、果糖、葡萄糖、半乳糖的峰面积标准曲线在0.2～6.4μg范围内均具有良好的线性关系，相关系数在0.9990 以上，加样回收率在96.62%～104.89%之间；相对标准偏差在0.76%～3.56%之间；建立的方法对五种糖的检测下限分别为60、100、100、60、80 ng。研究表明本方法灵敏、准确，可用于大枣中可溶性糖含量的质量控制与分析。 关键词：高效液相色谱法；蒸发光散射检测器；大枣；可溶性糖 中图分类号：R284.1 文献标识码：A 文章编号： Analysis of Carbohydrates in Ziziphus Jujuba Extraction by High Performance Liquid Chromatography with Evaporative Light Scattering Detector Yiming.Gaghafu，Xin Chen，Liying Zheng，Qilei Yin，Ruirui He，Xianwang Zhan， Yaoxin Wang，Xihui He，Nanyin Han* (School of Pharmaceutical Sciences, Peking University, Beijing P.R.China 100191)[基金项目] 与山西省华安母枣有限公司合作的重大开发项目 [基金项目] 与山西省华安母枣有限公司合作的重大开发项目 [通信作者] 韩南银*, 副教授, 硕士生导师，北京大学药学院，主要研究方向：药物分析 Abstract : A high performance liquid chromatography method with evaporative light scattering detector( ELSD) for the determination of five carbohydrates ( rhamnose, fructose, arabinose, glucose，galactose) in Jujuba extractjion was estabilished. The samples were separated by GRACE HYPERLINK /Prevail/10222424253.html \t _blank Prevail carbohydrate ES column using acetonitrile/water(72/28,v/v) as the mobile phase at a flow-rate of 1.0 mL /min . The optimum settings of the ELSD parameters:drift tube temperature was 80 ℃ and air carrier gas flow-rate was 2.00 mL /min. The results demonstrsted that the linear range was 0.2～6.4μg with the detecting limits ranged from 60 to100 ng. The recoveries were reproducible, ranging from 96.62% to 104.89%. The method is accurate, rapid and could be applied for the quantitative analysis of soluble carbohydrates in Jujube. Keywords: High performance liquid chromatography; Evaporative light scattering detector; Jujube; Carbohydrates Chinese Library Classification(CLC): R284.1 Document code?: A