金粉蕨素对甲萘醌损伤人脐静脉内皮细胞的保护作用-北京大学学报.PDF

10 Chinese Journal of Pathophysiology 2003, 19(1): 10- 13 [ ] 1000- 4718(2003)01- 0010- 04 * 郭 玉, 朱炳阳, 严奉祥, 黄红林, 廖端芳 ( , 421001) [ ] :: - - ,; MTT ,; NO /NO 2 3 ;Western blot (eNOS) ( caveolin- 1) : - - , NO /NO , eNOScaveolin 2 3 - 1: caveolin- 1/ eNOS [] ; ; K; [] R363 [] A ( onychin) (O2 ), (Sinopteridaceae)( Onychium ching) [3] ( 1mmol/L) ; M199 ( Onychium lucidum) Sigma ;NO (flavanone glycoside), ; caveolin- 1eNOS [1] HRP Santa Cruz; BCA (vascu (BCA protein assay reagent)PIERCE lar endothelial cell,VEC) (nitric 2 oxide,NO), 2 1 人脐静脉内皮细胞( human umbilical vein en dothelial cell, HUVEC) 的培养 , , ,PBS ,5- (nitric oxide synthase,NOS) N 10 mL 0 1%( ) ,37 20 min, - - L- (N- L- A) , PBS , , [2] M199 ( 20%25! NO PGI , 2 4 , 10 U/L250 mg/L 20 mg/L , caveolin 90 mg/L ),800 r/min - 1eNOS , 5 min;, M199 , ; 24 h , 1; , 0 25%