基于影像的脑胶质瘤原位移植瘤模型的建立Establishmentofaglioma.PDFVIP

基于影像的脑胶质瘤原位移植瘤模型的建立 季鸣，王丽嫄，吕元皓，来芳芳，陈晓光* （中国医学科学院、北京协和医学院药物研究所，天然药物活性物质与功能国家重点实验室， 北京 100050 ） 摘要：脑肿瘤原位移植瘤模型是临床前评价抗脑瘤药物效果的有效手段。本 研究利用荧光素酶和红色荧光蛋白双标记的人脑胶质瘤细胞 U87MG- mCherry-luc ，构建了基于影像的裸鼠脑原位移植瘤模型，利用已 的抗肿瘤药 物替莫唑胺，结合小动物核磁共振成像系统 （MRI ）、活体成像系统 （IVI ）等影 像技术验证了该模型的可行性。结果表明，该模型可有效地评价药物抗脑胶质瘤 的效果。 关键词：脑胶质瘤；原位移植瘤模型；影像技术 中图分类号：R966 文献标识码: A 文章编号：0513-4870(2016) Establishment of a glioma orthotopic xenograft model based on imaging technology JI Ming, WANG Li-yuan, LV Yuan-hao, LAI Fang-fang, CHEN Xiao-guang* (State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China) Abstract: Orthotopic xenograft model of human brain cancer cell is a good preclinical model for antitumor compound evaluation . In the present study, an orthotopic xenograft model of U87MG-mCherry-luc in Balb/c nude mice was established and monitored via imaging technology including magnetic resonance imaging (MRI), in vivo imaging (IVI) and micro-CT. Furthermore, the model was investigated by a positive drug temozolomide. Our data indicated that integrated 收稿日期: 2016-01-28; 修回日期: 2016-04-26 基金项目: 国家自然科学基金青年科学基金资助项目 ） *通讯作者 Tel / Fax: 86-10 E-mail: chxg@imm.ac.cn imaging technology including MRI, IVI and micro-CT in orthotopic xenograft model can facilitate monitoring of cancer progression and evaluate drug antitumor activity against glioma. Key word: glioma; orthotopic xenograft; imaging technology 脑胶质瘤（glioma ）是最常见的颅内恶性肿瘤，约占所有恶性脑肿瘤的80%， 具有发病率高、复发率高、死亡率高和治愈率低等特点。胶质瘤的治疗是手术、 ［ ］ 1,2 放疗和化疗为主的综合治疗 。替莫唑胺是新一代口服烷化剂，能透过血脑屏 ［ ］