己烯雌酚对小鼠睾丸发育的影响-基础兽医专业论文.docxVIP

己烯雌酚对小鼠睾丸发育的影响摘要：己烯雌酚(Diethylstilbestr01 己烯雌酚对小鼠睾丸发育的影响 摘要：己烯雌酚(Diethylstilbestr01 DES)是在畜牧业生产中得到广泛应用 人工合成的激素。在己烯雌酚长期使用过程中的研究发现，己烯雌酚具有生殖毒 性为主的严重毒副作用。 本试验第一部分就己烯雌酚对胎鼠睾丸发育的影响作以研究：试验分别设正 常组、对照组、DES 2微克组、DES 4微克组、DES 8微克组，各组母鼠从怀孕 第10日至第18日分别连续皮下注射，取出胎鼠睾丸。经切片HE染色观察并应 用免疫组织化学方法检测雄性胎鼠睾丸中增殖细胞核抗原(proliferating cell nuclear antigen PCNA)的表达情况。发现较大剂量韵DES作用如8微克、4微 克组均可引起睾丸生精细胞、支持细胞间质细胞形态、捧列结构上的变化，同时 可导致睾丸中PCNA含量降低。提示DES会抑制胚胎期睾丸细胞的增殖，从而影 响睾丸的发育，破坏皋丸的组织结构，并进而影响睾丸劝能的执行。 本试验第二部分就高效液相色谱法、荧光分光光度计法、毛细管电泳法三种 检测方法作比较。旨在寻找一种高效、快速、可行的动物组织中己烯雌酚残留检 测方法。试验表明高效液相色谱法(High Performance Liquid Chromatography HPLC)的最小检出量为6。25×IO-3mg·ml一，荧光光度法(Fluorescencespect rophotometer FS)的最小检出量8．04×10。mg·ml～，毛细管区带电电泳法 (caoillm-y zone electrophoresis CZE)的检测所用时间为4min。具有较快 的检测速度、仪器便宜、简便、快速、准确、试荆用量小、废液容易处理、污染 小。因此从国情出发，CZE法在基层实际检测中是一种有效、可行的检测方法。 关键词己烯雌酚睾丸增殖细胞核抗原高效液相色谱荧光分光光度计 毛细管区带电电泳胎鼠 Effects Effects DiethyIsti IbestroI Jof on orchi0 deve I opment of mouse Abstract DES iS artificially produced and widely used hormone in stockpreeding production．In the long period of using DES，its serious hazard to the genital system had been found． In the first part of experiment the effects of DES on orchic development of mouse was studied．the control group，DES 2lag ones，DES 4p．g ones，DES 8 lag ones was selected,each group was iaiected under skin from 10 days to 18 days，at last the testicle of embryo WaS collected and observed in the H．E stained preparation，expression ofPCNA in testicle was detected with immunohistochemistry．The results followed as：a massive dose of DES such as 4lag,8雌could change shape and s蚋比tIlre of spermatogenic ceU and interstitial cells，also caused the decreased content of PCNA．All above indicated the DES could inhibit the proliferation of orchic cells in embryo，then influenced the orchic development,damaged the orchic stracturc。ultiamtely effecmd on the func曲n of testicle。 In the second part of experiment the three methods of HPLCYS,CZE Was compared to find out a highly sensitive、quick and feasible met