不结球白菜波里马胞质雄性不育系.PDF

( ) 南京农业大学学报 　1997 ,20 3 : 36～43 　 　 Journal of N anjin g A gricult ural U niversity 不结球白菜波里马胞质雄性不育系 花药发育的细胞形态学研究 杨晓云 　曹寿椿 (南京农业大学蔬菜研究所 , 南京 210095) ( ) 摘要 　以不结球白菜波里马胞质雄性不育系 Pol cms 及相应保持系为试材 ,从显微和亚显微结构两个层 次对花药发育进行对比研究。结果表明 :不结球白菜 Pol cms 花药发育受阻于孢原细胞分化期 ,没有孢原 细胞分化 ,不形成药室。不育系经低温作用后 ,能产生微量花粉 ,部分花药能形成孢原细胞 ,并且能经过造 孢细胞、花粉母细胞、四分体、单胞花粉、二胞花粉等阶段而发育为成熟花粉。超微结构观察表明 :低温诱 导不育系形成的部分花粉在发育过程中的亚显微结构与保持系正常花粉具有不同的特点。 关键词 　不结球白菜 ; 波里马胞质雄性不育 ; 花药发育 ; 温度敏感 ; 细胞形态学 中图分类号 　S634301 CYTOMORPHOLOGICAL RESEARCH ON ANTHER DEVELOPMENT OF POL CMS IN NONHEADING CHINESE CABBAGE ( B RASSICA CAM P ES TRIS L. SSP. CHIN ENSIS MAKINO) Yang Xiaoyun and Cao Shouchun ( Institute of Vegetable Sciences , Nanjin g Agric Univ , Nanjin g 210095) ABSTRACT 　Cytomorphology of anther development of Pol cms in nonheading Chinese cabbage has been stud ied with light and electron microscopes. The observations showed that the development of the cms anthers was inhibited at the stage of archesporial cell. There was no differentiation of archesporial cell , and no anther sac formed. The male sterile plant produced some pollen under low temperature. Archesporial cells were formed in some anthers , and developed to mature pollen through the every stage of sporogenous cell , microspore mother cell , tetrad , uninucleate pollen and twocelled pollen. Ultrastructurally , there were still some differences between temperatureinduced pollen and normal fertile pollen either at the stage