杨梅醇和5-对氟苄氧基杨梅醇制备和其对人肝癌HepG2细.PDFVIP

杨梅醇与 5-对氟苄氧基杨梅醇的制备及其对人肝癌HepG 细胞活性的 2 影响  童晔玲，戴关海* ，任泽明，陈璇，聂晓静，杨锋(浙江省中医药研究院，杭州 310007) 摘要：目的 体外观察杨梅醇与5-对氟苄氧基杨梅醇(myricanol 5-fluorobenzyloxy ether ，5FEM)对人肝癌细胞HepG2 活性 的影响。方法 制备杨梅醇与5FEM ，杨梅醇(25 ，12.5，6.25 μg·mL1) 、5FEM(25 ，12.5，6.25 μg·mL1)作用于HepG2 细 胞，实时细胞分析系统记录96 h 细胞指数，观察杨梅醇与5FEM 对HepG2 细胞增殖和迁移的影响。结果 杨梅醇与5FEM 对 HepG2 细胞的增殖和迁移均有较好的抑制作用，且呈现较好的量效关系，相等剂量下，5FEM 的作用优于杨梅醇。结 论 杨梅醇与5FEM 能有效抑制HepG2 细胞的增殖和迁移。 关键词：杨梅醇；5-对氟苄氧基杨梅醇；人肝癌HepG2 细胞；细胞增殖；细胞迁移 中图分类号：R285.4 文献标志码：A 文章编号：1007-7693(2018)03-0375-04 DOI: 10.13748/ki.issn1007-7693.2018.03.014 引用本文：童晔玲，戴关海，任泽明，等. 杨梅醇与 5-对氟苄氧基杨梅醇的制备及其对人肝癌 HepG2 细胞活性的影响[J]. 中国现代应用药学, 2018, 35(3): 375-378. Preparation of Myricanol and Myricanol 5-fluorobenzyloxy Ether and its Effect on the Activity of HepG2 Cells * TONG Yeling, DAI Guanhai , REN Zeming, CHEN Xuan, NIE Xiaojing, YANG Feng(Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310007, China) ABSTRACT: OBJECTIVE To observe the activity of myricanol and myricanol 5-fluorobenzyloxy ether (5FEM) on liver cancer of cell HepG2 in vitro. METHODS Myricanol (25, 12.5 and 6.25 μg·mL1) and 5FEM (25, 12.5 and 6.25 μg·mL1) were prepared to act on HepG2 cells, real time cellular analysis was used to record 96 h cell index (CI) to observe the effect of Myricanol and 5FEM on the proliferation and migration of HepG2 cells. RESULTS Myricanol and 5FEM could inhibit the proliferation and migration of HepG cells, showing a dose-effect curve. The effect of 5FEM was better than myricanol at the 2 same dose. CONCLUSION Myricanol and 5FEM can effectively inhibit the proliferation and mig