微乳体系中脂肪酶催化棕榈酸制备生物柴油和其工艺优化.docVIP

微乳体系中脂肪酶催化棕榈酸制备生物柴油及其工艺优化 朱广琪 李芳浩 刘妍 王彩娜 谈忠琴 韩晓祥 （浙江工商大学应用化学系，杭州 310018） 摘 要 以十二烷基苯磺酸（Dodecyl benzenesulfonic acid, DBSA）+TX-100/环己烷/1-丁基-3-甲基咪唑四氟硼酸盐（BmimBF4）微乳体系为反应介质，Candida rugosa脂肪酶为催化剂，进行棕榈酸与乙醇酯化反应制备生物柴油，并考察离子液体用量、醇酸摩尔比、脂肪酶用量、反应温度、反应时间等因素对棕榈酸乙酯产率的影响。在单因素实验的基础上，根据中心组合 Box-Benhnken 实验设计原理，采用响应面分析法对生物柴油制备工艺进行优化。结果表明生物柴油制备的最佳工艺条件为：离子液体BmimBF4用量30%，醇酸摩尔比6.3:1，脂肪酶用量为棕榈酸质量的14%，反应温度34 ℃时，反应时间3.0 h，此条件下，棕榈酸乙酯的产率为97.5%，该结果与模型预测值基本相符。 关键词 离子液体微乳体系 脂肪酶 棕榈酸乙酯 响应面法 中图分类号：TQ645 文献标识码：A 文章编号： Optimization and Preparation of Biodiesel from Palmitate Acid using Candida Rugosa Lipase as Catalyst in Microemulsion System Zhu Guangqi Li Fanghao Liu Yan Wang Caina Tan Zhongqin Han Xiaoxiang (Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310018) Abstract Biodiesel was prepared via esterification of palmitate acid and ethanol in the dodecyl benzenesulfonic acid (DBSA) +TX-100/ cyclohexane/ 1-Butyl-3-methylimidazolium tetrafluoro- borate (BmimBF4) microemulsion system with Candida rugosa lipase as catalyst. The effects of several parameters such as the amount of ionic liquid, molar ratio of reactants, the amount of lipase, reaction temperature and reaction time on the yield of ethyl palmitate were investigated. On the basis of single factor experiments, the preparation process of biodiesel was optimized by response surface methodology (RSM) based on the Box–Behnken design (BBD). Accordingly, a maximum ethyl palmitate yield of 97.5% was obtained under the optimal conditions: amount of ionic liquid BmimBF4 30%, ethanol/acid molar ratio of 6.3, the amount of lipase to palmitate acid 14%, reaction temperature 34 ℃ and reaction time 3.0 h. These results were basically agreement with predicted value by a mathematical model. Key words ionic liquid microemulsion system, lipase, ethyl palmitate, response surface methodology ——————— 收录日期：2017-09-14 作者简介：朱广琪，女，1993年出生，硕士研究生，食品科学与工程 通讯作者：韩晓祥，男，1974年出生，副教授，食品化学。电话Email：hxx74@126.com