万寿菊叶精油的提取及化学成分分析-食品科学.PDF

※分析检测 食品科学 2010, Vol. 31, No. 18 359 李 健，宋帅娣，刘 宁，陈姝娟 (哈尔滨商业大学食品工程学院，黑龙江 哈尔滨 150076) 采用水蒸气蒸馏法提取黑龙江产万寿菊叶精油，通过单因素和正交试验，研究万寿菊叶精油提取的最佳 工艺条件。结果表明：最佳工艺条件为料液比 1:6、蒸馏时间 5h 、加热温度 1 1 0 ℃，此条件下精油的产率为 0.0944% 。采用气相色谱- 质谱法对精油的化学成分进行分析，共分离出 31 个峰，鉴定出其中的 29 个化合物，占 总峰面积的93.55% 。主要成分有异松油烯(37.02%)、2- 异丙基 -5- 甲基-3- 环己烯 -1- 酮(14.08%)、柠檬烯(13.08%)、 β- 罗勒烯(8.78%) 、石竹烯(4.18%) 。 万寿菊；精油；水蒸气蒸馏；化学成分；气相色谱 - 质谱 　　 Extraction and Chemical Composition Analysis of Essential Oil from African Marigold (Tagetes erecta L.) Leaves LI Jian ，SONG Shuai-di，LIU Ning ，CHEN Shu-juan (College of Food Engineering, Harbin University of Commerce, Harbin 150076, China) Abstract：The steam distillation extraction of essential oil from African marigold leaves was optimized for achieving maximum oil yield using single factor and orthogonal array design methods, and chemical composition analysis of the extracted oil was carried out using gas chromatography-mass spectrometry (GC-MS). The optimal conditions for oil extraction were found as follows: material-liquid ratio 1:6; distillation duration 5 h; and heating temperature 110 ℃, and the resultant oil yield was up to 0.0944%. The GC-MS analysis showed that 31 peaks were separated, and 29 of them were identified, accounting for 93.55% of the total peak area, and the main components were cyclohexene (37.02%), 2-isopropyl-5-methyl-3-cyclohexen-1-one (14.08%), limonene (13.08%), 3,7-dimethyl-1,3,6-octatriene (8.785%) and caryophyllene (4.18%). Key words ：African marigold (Tagetes erecta L.) ；essential oil ；steam distillation ；chemical composition ；GC-MS 中图分类号：TQ651.2 文献标识码：A 文章编号：1002-6630(2010)18-0359-04 万寿菊学名 Tagetes erecta L. ，为菊科万寿菊属一 1 材料与方法 年生草本植物，原产于热带美洲