丝胶蛋白及其木瓜蛋白酶酶解产物的保湿性与抗-安徽农业大学学报.PDF

安徽农业大学学报, 2016, 43(5): 682-685 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x028 网络出版时间：2016-9-29 10:30:16 [URL] /kcms/detail/34.1162.S1030.056.html 丝胶蛋白及其木瓜蛋白酶酶解产物的保湿性与抗氧化活性 叶崇军，李 冰，陈复生* (安徽省农业科学院蚕桑研究所，合肥 230061) 摘 要：以全天然丝胶茧为原料提取丝胶蛋白，经木瓜蛋白酶酶解，考察丝胶蛋白及其酶解产物的保湿性，并 从清除 DPPH 自由基能力、总抗氧化能力、抑制烃基自由基能力和抗超氧阴离子自由基能力等方面分析抗氧化活 性，为开发利用优质的丝胶蛋白资源的更广泛应用提供理论依据。结果表明，丝胶蛋白经木瓜蛋白酶酶解后获得5 ～ 12 kDa 的酶解产物，其优良的保湿性能优于丙二醇；丝胶蛋白及其酶解产物均具有较好的抗氧化活性，其中以酶 解产物的抗氧化活性更强。抗氧化能力与浓度呈明显的正相关性。 关键词：丝胶蛋白；木瓜蛋白酶；酶解产物；保湿性；抗氧化活性 中图分类号：S881.3 文献标识码：A 文章编号：1672−352X (2016)05−0682−04 Moisture retention and antioxidant activities of silk sericin and its enzymatic hydrolysate YE Chongjun, LI Bing, CHEN Fusheng (The Sericultural Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230061) Abstract: In order to provide a theoretical basis for more extensive application of the development and utili- zation of high-quality resources of sericin ，we used all natural sericin as raw materials ，extracting sericin, hy- drolyzing silk sericin by papain, to study the moisture retention property and antioxidant activity of silk sericin and its enzymatic hydrolysate from the aspects of scavenging capacity on DPPH radical, total antioxidant ability, scavenging effect on hydroxyl radi, anti-hydroxyl free radical ability. The results showed that, the molecular weight of enzymatic hydrolysate of sericin by papain was from 5 to 12 kDa. The moisturizing property of the en- zymatic hydrolysate was better than that of propylene glycol. Silk sericin and its enzymatic hydrolysate were found to have good antioxidant activity, of which the hydrolysates were stronger. There was a positive correlation between antioxidant capacity and its concentration. Key