山核桃林地土壤真菌群落结构研究-浙江林业科技.PDF

浙 江 林 业 科 技，2018 ，38 （5 ）：67 －72 J Zhejiang For Sci Technol doi:10.3969/j.issn.1001-3776.2018.05.011 山核桃林地土壤真菌群落结构研究 李 皓，董建华，袁紫倩，胡俊靖，赵伟明 （杭州市林业科学研究院，浙江 杭州 310022 ） 摘要：采用 16S rDNA 高通量测序技术分析杭州临安区正常生长和不良生长状况 2 种山核桃 Carya cathayensis 林 地土壤真菌群落结构。结果表明，生长不良山核桃林地与正常林地真菌优势类群相同，不同样地中子囊菌门 Ascomycota 、接合菌门 Zygomycota 、担子菌门 Basidiomycota 均为优势类群，但相对丰度不同，正常林地土壤中 担子菌门和接合菌门的相对丰度高于生长不良林地。在属的分类水平上，正常林地土壤中小画线壳 属 Monographella 、镰刀霉属 Fusarium 和土赤壳属 Ilyonectria 的相对丰度明显低于生长不良林地。生长不良林地土壤 真菌多样性高于正常林地，Shannon-Wiener 指数，ACE 指数，Chao1 指数与土壤 pH 之间呈显著负相关 （P0.05 ）， 与有机质、碱解氮和交换性酸显著正相关（P0.05 ）。 关键词：山核桃；土壤真菌；群落结构；16S rDNA 测序 中图分类号：S664.9 文献标识码：A 文章编号：1001-3776 （2018 ）05-0067-06 Soil Fungi Community Structure in Carya cathayensis Forest LI Hao ，DONG Jian-hua ，YUAN Zi-qian ，HU Jun-jing ，ZHAO Wei-ming （Hangzhou Forestry Academy, Hangzhou 310022, China ） Abstract: Soil samples in 0-20 cm layers were collected in August of 2016 from six plots at normal and unhealthy growth Carya cathayensis stands in Lin’an, Zhejiang province. Determinations on chemical properties were implemented and fungi community structure was analyzed by 16S rDNA sequencing. The results indicated that dominant phylums and genera were similar in the two different kinds of soil, including Ascomycota, Zygomycota and Basidiomycota, but relative abundance was different. Relative abundance of Basidiomycota and Zygomycota in normal growth plots was higher than that in unhealthy ones. Relative abundance of Monographella , Fusarium and Ilyonectria in unhealthy plots was higher than that in normal ones. Correlation analysis demonstrated that Shannon-wiener, ACE and Chao1 index had significant negative correlation with pH and