基于金针菇全基因组的赖氨酸合成途径关键酶分析.PDF

微生物学通报 Oct. 20, 2016, 43(10): 2225−2233 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.160379 研究报告 基于金针菇全基因组的赖氨酸合成途径关键酶分析 * 刘建雨 王瑞娟 张丹 尚晓冬 谭琦 ( 201403) 摘 要：【目的】以金针菇全基因组测序数据为基础研究其L-赖氨酸的从头合成途径及其关键 基因。【方法】采用Illumina Hiseq2000 和Roche454 FLX+ 两种方法完成金针菇单孢菌株Dan3 的基因组测序，基于全基因组序列筛选金针菇中赖氨酸生物合成的关键基因，并分析这些基因 编码的蛋白质基本理化性质；预测其亚细胞定位情况及蛋白的二级结构。【结果】金针菇Dan3 全基因组序列长度为34.17 Mb ，预测到8 个参与α-氨基己二酸途径的关键基因，这些基因都含 有多个内含子和外显子，二级结构主要由α-螺旋和无规则卷曲组成，有3 个蛋白定位于线粒体。 【结论】金针菇是通过α-氨基己二酸途径合成赖氨酸，基因组中预测到与该途径相关的几乎所 有的酶。 关键词：金针菇，赖氨酸生物合成，α-氨基己二酸途径 Analysis of genes related to lysine biosynthesis based on whole genome of Flammulina velutipes * LIU Jian-Yu WANG Rui-Juan ZHANG Dan SHANG Xiao-Dong TAN Qi (Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China) Abstract: [Objective] To analyze lysine biosynthetic pathway and related genes based on the whole genome of Flammulina velutipes . [Methods] Genome sequence of the monosporous strain Dan3 was sequenced through Illumina Hiseq2000 and Roche454 FLX+. Key genes related to lysine biosynthesis pathway were screened based on the whole genome. Basic physical and chemical properties of these proteins were analyzed, and the sub