响应面法优化桔青霉产核酸酶P1培养基-食品科学.PDFVIP

※生物工程 食品科学 2011, Vol. 32, No. 17 283 P1 1 1, 2 2 2 2 喻 晨 ，张亚雄 * ，赵 劼 ，朱昌雄 ，杨海珍 ，姚 鹃 (1.三峡大学生物工程重点实验室，湖北 宜昌 443002 ；2.安琪酵母股份有限公司，湖北 宜昌 443003) 采用部分析因试验设计(FFD) 、中心组合实验设计(CCD)和响应面分析法(RSM)对桔青霉产核酸酶 P1 培养 基进行优化研究。FFD 结果表明：硫酸锌、酵母粉、复合磷酸盐 3 个因素显著影响桔青霉发酵产核酸酶 P1 ，利 用最陡爬坡实验(SAD)使得 3 个显著因素的水平取值逼近最大响应区域，通过CCD 和RSM 确定了 3 个显著性因素的 最优水平，得到桔青霉发酵产核酸酶 P1 的最优培养基配方：葡萄糖4% 、酵母粉0.681% 、玉米浆0.4% 、黄豆饼 粉 0.45%、硫酸锌0.042%、硫酸镁0.06%、氯化钙0.06%、复合磷酸盐(m(磷酸二氢钾):m(磷酸氢二钾)=1:1)0.068% 。 验证实验表明，优化后的培养基发酵酶活力达到 563U/mL ，比原培养基(202U/mL)提高了 64% 。 核酸酶 P1 ；桔青霉；培养基优化；响应面法 Medium Optimization for Production of Nuclease P1 in Penicillium citrinum by Response Surface Methodology 1 1, 2 2 2 2 YU Chen ，ZHANG Ya-xiong *，ZHAO Jie ，ZHU Chang-xiong ，YANG Hai-zhen ，YAO Juan (1. Key Laboratory of Bioengineering, China Three Gorges University, Yichang 443002, China； 2. Angel Yeast Co. Ltd., Yichang 443003, China) Abstract ：Fractional factorial design (FFD), central composite design (CCD) and response surface methodology (RSM) were used to optimize the culture medium for nuclease P1 production by Penicillium citrinum. The results of fractional factorial design showed that ZnSO4, yeast extract and composite phosphate had a significant effect on nuclease P1 production. The steepest ascent design (SAD) made three significant factors approach the maximum response area. Three significant factors were optimized by CCD and RSM. The optimized culture medium was composed of 4% glucose, 0.681% yeast extract, 0.4% corn syrup, 0.45% soybean meal, 0.042% ZnSO4 , 0.06% MgSO4 , 0.06% CaCl2, an