浊度法抗生素效价测定仪的校准技术及其应用-中国抗生素杂志.PDF

中国抗生素杂志2018年1月第43卷第1期 . 69 . 文章编号：1001-8689(2018)01-0069-04 浊度法抗生素效价测定仪的校准技术及其应用 丁峰元 (河南省计量科学研究院，郑州450008) 摘要：根据浊度法抗生素效价测定仪的工作原理和结构组成，编制了校准方法并研制相应的校准设备。使用数字温度计 可实现仪器控温性能的温度偏差、温度均匀度和温度波动度等3个技术指标的校准，以上3个技术指标分别为± 1.0 ℃、1.0 ℃和 ±0.5℃。使用光谱中性滤光片可实现仪器吸光度测量性能的零点漂移、示值漂移、示值误差、测量重复性和通道差异等5个技术 指标的校准，以上5个技术指标分别为±0.005、±0.005、±0.03、0.005和0.03 。经验证，校准方法有效，可确保浊度法抗生素效价 测定仪测量结果的溯源性和可比性。 关键词：浊度法抗生素效价测定仪；校准技术；控温性能；吸光度示值性能 中图分类号：R927.11 文献标志码：A Calibration techniques and applications of antibiotic potency analyzers based on the turbidimetric method Ding Feng-yuan (Henan Institute of Metrology, Zhengzhou 450008) Abstract According to the working principle and the instrument structure of antibiotics potency analyzer based on the turbidimetric method, calibration techniques were programmed and calibration applications were designed. Three parameters of the temperature control performance, including temperature bias, temperature evenness, and temperature fluctuation, were calibrated using digital thermometers. The technical indexes of the three parameters mentioned above were ±1.0 ℃, 1.0℃, and ±0.5℃, respectively. Five parameters of the absorbance indication performance, including zero drift, indication drift, indication error, measurement repeatability, and channel difference, were calibrated using spectrum neutral filters. The technical indexes of the five parameters mentioned above were ±0.005, ±0.005, ±0.03, 0.005, and 0.03, respectively. The uncertainty in the measurement of the digital thermometer was U=0.3 ℃(k=2). The