凝集素芯片在糖链结构变化检测中的应用.pdfVIP

· 316 · 生命的化学, 2013, 33(3): 316-323 《生命的化学》2013 年33 卷3 期 综述 凝集素芯片在糖链结构变化检测中的应用 1,2 1,2 1,2 1,2* 程莉 ，周树敏 ，郭书娟 ，陶生策 1 ( 上海交通大学系统生物医学研究院，上海200240； 2上海市肿瘤研究所，癌基因及相关基因国家重点实验室，上海200240) 摘要：细胞表面的糖蛋白及糖脂在许多生物学过程中均发挥着重要作用，如免疫保护、细胞通讯、炎 症产生等。近年研究发现糖基化的改变与许多疾病如肿瘤的发生发展及转移密切相关。凝集素是一类 能够与糖链结构特异性结合而又不具有糖链合成、转移和催化活性，且非免疫来源的蛋白质，已被广 泛的应用于糖免疫学研究。凝集素芯片可快速、高通量分析生物样本糖构型，是一种检测细胞表面糖 链整体变化的通用平台。本文对凝集素芯片的基本概念、技术发展及其在检测细胞表面糖谱和临床样 本分析等方面的应用进行综述，并对其今后的发展方向进行展望。 关键词：凝集素芯片；糖基化；活细胞表面糖谱；糖蛋白 Glycan profiling based on lectin microarray CHENG Li1,2, ZHOU Shumin1,2, GUO Shujuan1,2, TAO Shengce1,2* 1 ( Shanghai Jiao Tong University, Shanghai Center for Systems Biomedicine, Shanghai 200240, China; 2 State Key Laboratory of Oncogenes and Related Genes, The Shanghai Cancer Institute, Shanghai 200240, China) Abstract: The cell surface glycoproteins and glycolipids paly very important roles in many biological processes, such as immunity-protection, cell-to-cell communication, and inflammation. Recent studies have showed that the glycosylation aberrations are highly associated with disease occurrence and progress, e.g., tumor development and metastasis. Lectins, which have long been used in glyco-immunology, are defined as a class of proteins of nonimmune origin that bind carbohydrates without modifying them. Lectin microarray provides a versatile platform for rapid high-throughput analysis of the glycan profiling of living cell surface. In this review, we summarize the basic concepts and the latest