一株珊瑚虫草菌丝培养物中天然色素的分离和高-安徽农业大学学报.PDFVIP

安徽农业大学学报, 2011, 38(4): 596-599 Journal of Anhui Agricultural University [DOI]CNKI：34-1162/S1625.021 网络出版时间：2011-06-24 16:25:42 [URL]/kcms/detail/34.1162.S1625.021.html 一株珊瑚虫草菌丝培养物中天然色素的分离 和高分辨质谱分析 王学新，王小董，耿广青，庄梦敏，罗飞飞，张 琛，胡丰林* (安徽农业大学微生物防治省重点实验室, 合肥 230036) 摘 要：在一次对虫生真菌天然色素筛选过程中，发现 1 株珊瑚虫草分离菌株 RCEF4022 的菌丝体含黄色色素。 采用半制备高效液相色谱方法从该菌甲醇提取物中分离纯化出 1 种橘黄色物质 P-1 。高分辨液质联用分析结果表明 P-1 的分子式为 C30H18O12 。经化学试剂显色反应以及波谱学方法鉴定，确定 P-1 为羟基蒽醌类色素 2,2,4,4,5,5-hexahydroxy-7,7-dimethoxy-1,1-bianthraquinone 。该色素化合物尚属首次从虫生真菌中分离得到。 关键词：珊瑚虫草; 天然色素; ESI-MS 中图分类号：S476.12 文献标识码：A 文章编号：1672−352X (2011)04−0596−04 Isolation and HPLC-HRMS analysis of a natural pigment from Mycelia of a strain of Cordyceps martialis WANG Xue-xin, WANG Xiao-dong, GENG Guang-qing, ZHUANG Meng-min, LUO Fei-fei , ZHANG Chen, HU Feng-lin (Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036) Abstract: During screening for natural pigments from entomogenous fungi, mycelia of a strain RCEF4022 of Cordyceps martialis were observed to contain a yellow pigment. An orange yellow pigment named P-1 was ex- tracted from the mycelia with methanol and prepared with semi-preparative HPLC （High Performance Liquid Chromatography ）. High resolution MS analysis revealed that the molecular formula of the pigment was C30H18O12. The pigment compound was identified by color development reaction, Ms and UV as a dimeric an- thraquinone 2,2,4,4,5,5-hexahydroxy-7,7-dimethoxy-1,1- bianthraquinone. This pigment was isolated from en- tomogenous fungi for the first time. Key words: Cordyceps martialis; natural pigment; ESI-MS 天然色素与人工合成色素相比，具有毒性低、