黄瓜低霜霉威残留性相关基因SDH的克隆及遗传转化-园艺学；蔬菜学专业毕业论文.docxVIP

目录参考文献．．43附录52攻读硕士学位期间发表的学术论文一53III万方数据CoNll孙iTSCoNTENTSAbstract in Chinese．IAbstract in English．IIII Introduction．．11．1 Purpose and significance ofthe experiment～11．2 National and abroad research summarization21．2．1 Summary ofpesticide residue一21．2．2 Research progress ofreducing pesticide residue一51．2．3 Research progress ofrelated genes on pesticide residue一71．2．4 Research progress on SDH gene一81．3 Research contents ofthis paper81．4 Technique route一92 Mateials and methods102．1 Experiment materials．1 02．1．1 Plant materials．．1 02．1．2 Main instruments．102．1．3 Test reagents，enzymes and strains102．2 The clone of Cucumber CsSDH gene．．1 12．2．1 Extraction of Total RNA1 l2．2．2 cDNA first strand synthesis．．1 12．2．3 The design and synthesis ofprimers．1 12．2．4 The PCR expanding of Cucumber CsSDH gene．122．2．5 Recycling and sequencing ofthe PCR product．．132．3 Sequence analysis of Cucumber CsSDH gene ．．．1 5 2．4 Expression analysis of Cucumber CsSDH gene under propamocarb processing1 62．5 Activity changes of Cucumber succinic dehydrogenase after propamocarb processing．．．．．．．1 6 2．6 Obtain ofCucumber CsSDHplant expression vector fragment．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．17 2．7 Construction of Cucumber CsSDH over-expression vector ．1 72．7．1 Recombinant plasmid and double enzyme digestion of PBll2 1 empty carrier1 72．7．2 Connection and transformation of plant expression vector and purpose gene segments．1 82．7．3 Identification ofthe recombinant plasmid ．1 8 2．7．4 Recombinant plasmid leading-in Agrobacterium tumefaciens by freeze-thaw method 1 9 2．7．5 PCR of recombinant plasmid and enzyme digestion identification in agrobacteriumtamefaciens192．8Cucumber CsSDH genetic transformation to Arabidopsis．192．8．1 Arabidopsis materails192．8．20btain of To seeds ofArabidopsis transgenic plants．20V万方数据东北农业大学农学硕士学位论文2．8．3Screening ofresistant seeds．202．8．40btain the mutant arabidopsis蛹aliana plants and extraction ofDNA．212．8．50btain of To seeds ofArabidopsis transgenic plants．2 12．8．6 Molec