黄淮海地区重组型大豆花叶病毒的侵染性与生物学特性分析-遗传学专业毕业论文.docxVIP

文库，通过双酶切手段将eDNA文库构建至pGADT7．SfiI载体上。检测结果 文库，通过双酶切手段将eDNA文库构建至pGADT7．SfiI载体上。检测结果 显示，文库容量约为3．2×106 efu，插入eDNA片段长度在0．75～2Kb之间，平 均长度大于1 kb，且多态性丰富，表明文库质量较好。 以上研究结果表明：重组型SMV在黄淮海地区分布普遍，在北京、安徽、河北 各省市均有发现。且重组型SMV与非重组型SMV基因组结构基本一致，但重组型 SMV的P1端是由SMV和BCMV在P1编码区重组而来的。重组型SMV与非重组 型SMV中的SC6亲缘关系最近，两者的致病性和侵染性是具有差异的，重组型SMV 在大豆植株内更具有侵染性，而致病性则较Sc6弱。高质量的感染重组型SMV本氏 烟eDNA文库的构建，为今后进～步研究重组型SMV的侵染机制奠定了物质平台。 关键词：大豆；重组型大豆花叶病毒；抗性鉴定；eDNA文库 万方数据 Pathogenicity Pathogenicity and biological characteristics of Recombinant Soybean Moasic Virus in the Huang-Huai-Hai Author：Lin Jing Major：Genetics Supervisor：Prof．Xie Lmg-qm Prof．Zhang Meng-chen Abstract Soybean Mosaic Vuus(SMV)is a worldwide disease．It occurs in most soybean production areas and seriously affects the production and economic value of soybean． Mutation and recombination are two most important reasons led to new virus appear,host range and pathogenic changes．Understanding pathogen information and determine the pathogenicity of pathogen play important roles in preventing and controlling virus pandemic．In 20 1 1，recombined SMV was first identified in Chongqing soybean production region by Yang et a1．However,until now systemic research has not been carried out on recombined SMV and this made the disease·resistant breeding facing new challenges．In this study，basing on the discovery of the new type SMV,we investigated the composition of SMV in soybean production regions of Huang-huai-hai．Further we isolated a recombined SMV strain，the whole genome of the recombined SMV strain was sequenced and its phylogenetic classification and status were analyzed．Several soybean cultivars were selected for resistance identification and pathogenicity and characteristics of recombinant SMV were compared with non-recombinant SMV strain．Furthermore，we constructed a eDNA library of the model crops Nicotiana benthamiana，which could systematic infected by recombinant SMV,for yeast two-hybrid and it will lay the material platform for further research on recombinan