油桃采后衰老与病害发生中品质与活性氧代谢相关性分析-食品科学.PDFVIP

244 2011, Vol. 32, No. 20 食品科学 ※包装贮运 杨宏伟，陈小燕，张 影，王友升 * ，李丽萍 (北京工商大学食品学院 北京市食品风味化学重点实验室，食品添加剂与配料北京高等学校工程研究中心， 北京 100048) 探讨油桃果实在采后衰老与病害发生中近果核和近表皮处的品质与活性氧代谢的相关性。结果显示油桃 采后衰老与病害发生中膜完整性、过氧化物酶( p e r o x i d a s e ，P O D ) 活性呈下降趋势，岀汁率、脂氧合酶 (lipoxygenase ，LOX)活性、H2 O2 含量呈升高趋势，过氧化氢酶(catalase ，CAT) 、抗坏血酸过氧化物酶(ascorbate peroxidase ，APX) 、多酚氧化酶(polyphenol oxidase ，PPO) 比活力呈先升高后下降的趋势。偏最小二乘回归分析 结果表明：膜完整性与岀汁率、H2 O2 、LOX 呈负相关，与 POD 、APX 呈正相关。通径分析得出 APX 、POD 、 PPO 对 H2O2 含量变化有强的负直接作用，其作用由强到弱为 APX ＞POD ＞LOX 。为进一步探讨相关酶在采后油 桃果实抵抗病原菌侵染过程中所起的作用提供理论依据。 油桃；衰老；病害；主成分分析；偏最小二乘回归；通径分析 Analysis of the Relationship between Quality and Active Oxygen Species Metabolism in Nectarine during Postharvest Ripening and Pathogenesis YANG Hong-wei ，CHEN Xiao-yan ，ZHANG Ying ，WANG You-sheng* ，LI Li-ping (Beijing Key Laboratory of Food Flavour Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China) Abstract：The relationship between fruit quality and active oxygen species metabolism near the epidermis and core of nectarine during postharvest ripening and pathogensis caused by artificial infection with Monilinia fructicola was analyzed. The results indicated that membrane integrity and peroxidase (POD) activities declined, juice rate, lipoxygenase (LOX) activities and H2O2 content increased, while the activities of catalase (CAT), ascorbate peroxidase (APX) and polyphenol oxidase(PPO) increased firstly and then decreased during postharvest ripening and pathogenesis. Partial least squares regression (PLSR) results iden