基于DPPH自由基清除能力的姜黄提取物抗氧化.pdf

- 360 - Drug Evaluation Research 第 34 卷 第 5 期 2011 年 10 月 基于 DPPH 自由基清除能力的姜黄提取物抗氧化活性评价 王笑晴 天津医科大学第二医院，天津 300021 摘 要：目的 建立高通量检测 DPPH 自由基清除能力的实验方法，对姜黄提取物抗氧化活性进行初步评价。方法 通过 测定抗坏血酸（维生素 C ）的 DPPH 自由基清除率曲线，以IC50 值作为评价试样清除 DPPH 自由基能力的指标，并将此应 用于测定姜黄醇提物和水提物清除 DPPH 自由基的能力。结果 测定波长 515 nm，抗坏血酸 DPPH 自由基清除能力在62.5～ 750.0 μg/mL 线性良好，方法可靠。姜黄醇提物的 DPPH 自由基清除能力（IC50 7.78 mg/mL ）明显强于水提物（IC50 14.84 mg/mL ）。结论 建立的 DPPH 自由基清除测定方法可靠、简便、灵敏，其高通量的快速检测方法可为抗氧化药物筛选提供 参考。 关键词：姜黄；DPPH 自由基；抗氧化活性；高通量；抗坏血酸；维生素C 中图分类号：R977.9 文献标志码：A 文章编号：1674 - 6376 (2011) 05 - 0360 - 04 Evaluation on anti-oxidant activity of Curcuma longa extracts based on DPPH free radical scavenging capability WANG Xiao-qing Second Affiliated Hospital, Tianjin Medical University, Tianjin 300021, China Abstract: Objective To establish a high-throughput method of DPPH free radical scavenging for evaluating the anti-oxidant activity of the extracts from Curcuma longa. Methods Taking IC50 as the evaluating criterion of DPPH free radical scavenging capability by determining the scavenging rate curves of ascorbic acid, the DPPH free radical scavenging capability of two extracts from C. longa was determined. Results At the wavelength of 515 nm, DPPH free radical scavenging rate of ascorbic acid and concentration could fit a good linearity (0.062 5—0.75 mg/mL), and the results revealed that the method is convenient and reliable. Under this condition, the DPPH free radical scavenging capability of alcohol extracts (IC50 7.78 mg/mL) was significantly stronger than that of water extracts (IC50 14.84 mg/mL) from C. longa. Conclusion The convenient and reliable method could be used to evaluate the anti-oxidant activity of other Chinese materia medica, as well as provide an approach and reference for high-throughput activity screening. Key words: Curcuma longa L.; DPPH free radical; anti-oxidant act