暗黑链霉菌PY.pdfVIP

植物保护学报Journal of Plant Protection ，2018 ，45 （4 ）：864-870 DOI ：10.13802/ki.zwbhxb.2018.2017087 暗黑链霉菌PY-1 活性产物分析及其对葡萄霜霉病 田间防效评价 臧超群 白元俊 张海东 谢瑾卉 林 英 梁春浩* （辽宁省农业科学院植物保护研究所，沈阳 110161） 摘要：为探究暗黑链霉菌Streptomyces atratus PY- 1 对葡萄霜霉病菌Plasomopara viticola 的抑制作 用，通过扫描电镜观察PY- 1发酵滤液对葡萄霜霉病菌孢子囊的影响，检测PY- 1 的活性物质种类， 并测定其对葡萄霜霉病的田间防效。结果表明，PY- 1发酵滤液能够导致葡萄霜霉病菌孢子囊和孢 子囊梗出现褶皱、破裂和畸形，进而使其丧失侵染功能。PY- 1 菌株代谢产物中包含几丁质酶、蛋白 酶、嗜铁素、1-氨基环丙烷- 1-羧酸脱氨酶、氰化氢、吲哚乙酸，不含纤维素酶。PY- 1 菌株对葡萄霜霉 病具有较好的田间防效，发酵原液对葡萄霜霉病的田间中期防效可达到89.17%以上，末期防效达 86.28%以上，比52.5%噁唑菌酮·霜脲氰2 000 倍液的防效略低，但显著高于58%甲霜·锰锌1 000 倍 液的防效；PY- 1 菌株发酵液稀释700 倍后对葡萄霜霉病的末期防效与甲霜锰锌1 000 倍液防效相 当。表明PY- 1 菌株具有研制防治葡萄霜霉病生防制剂的潜力。 关键词：葡萄霜霉病菌；暗黑链霉菌；活性产物；田间防效 Study on bioactive metabolite of Streptomyces atratus PY-1 and the field control efficiency against grapevine downy mildew Zang Chaoqun Bai Yuanjun Zhang Haidong Xie Jinhui Lin Ying Liang Chunhao* （Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, Liaoning Province, China ） Abstract: To explore the inhibitory effect of Streptomyces atratus PY- 1 against Plasomopara viticola, the effects of PY- 1 strain by filtered broth on sporangium of P. viticola was tested, the bioactive sub- stances were detected using specific medium and the biocontrol effect was evaluated on field experi- ments. The results showed that sporangium and sporangiophore of pathogen were wrinkled, wizened and fracture after treated with extracts of PY-1 filtered broth. The metabolite of PY-1 strain included chi- tinase, protease, siderophore, 1-aminocyclopropane-1-caboxylate deaminase, hydrogen cyanide, indole- 3-acetic acid, but excluded cellulase. The original fermentation solution o