柱前衍生化.pdf

160 2016, Vol.37, No.14 食品科学 ※安全检测 柱前衍生化-高效液相色谱法测定 地沟油中的脂肪醛 沈卫阳，王洁琼，王 婉，于清峰，肖 莹* （中国药科大学理学院，江苏南京 211198 ） 摘  要：研究利用油脂中的不饱和脂肪酸在提炼过程中发生脂质过氧化反应、产生低分子脂肪醛的特点，采用柱前 衍生化-高效液相色谱法，以2,4-二硝基苯肼为醛的衍生化试剂、2- 甲基吡啶硼烷为还原剂，通过测定脂肪醛类物质 来检测食用油中是否掺入了地沟油。结果表明：与食用油相比，精制地沟油中甲醛、乙醛的含量显著增加，可据此 判断样品是否为地沟油。该方法专属性强、灵敏度高、准确性好，极大地优化了地沟油的检测与鉴定流程。 关键词：地沟油；脂肪醛；2,4-二硝基苯肼；高效液相色谱 Determination of Aliphatic Aldehydes in Waste Cooking Oil by Pre-Column Derivatization-HPLC Method * SHEN Weiyang, WANG Jieqiong, WANG Wan, YU Qingfeng, XIAO Ying (School of Science, China Pharmaceutical University, Nanjing 211198, China) Abstract: By taking advantage of lipid peroxidation of unsaturated fatty acids and consequent generation of a variety of highly reactive small molecular aliphatic aldehydes during the refining of waste cooking oil, a simple and highly sensitive method was established based on the precolumn derivatization of aliphatic aldehydes utilizing 2,4-dinitrobenzene hydrazine (DNPH) followed by high performance liquid chromatography (HPLC). Further, high sensitivity was obtained by adding 2-picoline borane (2-PB) as a reducing agent to convert cis- and trans-isomers into the same structure. Compared with edible oil, the contents of formaldehyde and acetaldehyde in refined waste oil increased significantly, providing an indicator to discriminate waste cooking oil. This method is specific, highly sensitive and accurate and greatly improves the procedure for detection and identification of waste cooking oil. Key words: waste cooking oil; aliphatic aldehyde; 2,4-dinitrobe