麻醉及手术创伤对老年大鼠认知功能及海马突触结构的影响-麻醉学专业毕业论文.docxVIP

兰州大学硕上学位论文麻醉及手术创伤对老年大鼠认知功能及海马突触结构的影响 兰州大学硕上学位论文 麻醉及手术创伤对老年大鼠认知功能及海马突触结构的影响 摘要 目的探讨麻醉及手术创伤对老年大鼠认知功能及海马突触结构和胆碱乙酰 转移酶表达的影响。 方法18月龄SD大鼠56只随机分为三组：对照组(C组，n=8)、麻醉组(A 组，n=24)、手术组(O组，n=24)，A、0组根据麻醉、手术后1天、3天和7天 一 粤 分为3个亚组A1、A3、A7组和01、03、07组，每亚组8只。在相应的时间点用 筘 Morris水迷宫测试老年大鼠的认知功能，免疫组织化学方法检测海马胆碱乙酰 转移酶的表达，电镜定量测量海马CA3区多形层的突触结构。 结果认知功能测试表明：Al、01、03组Morris水迷宫测试潜伏期及游泳 距离比对照组明显延长(均为PO．05)，01组潜伏期与A1组比较明显延长(P O．05)，游泳距离无明显差异，07组潜伏期及游泳距离与C组的差异无统计学 意义；01、03组胆碱乙酰转移酶表达减少(均为尸O．01)；01、03组海马CA3 区多形层突触间隙宽度加大变宽(均为PO．01)，突触后膜致密物厚度变薄(均 为尸0．01)，突触活性区长度缩短(均为P0．05)，突触界面曲率减小(均为 尸O．01)，此外穿孔性突触百分率也有下降，07组恢复正常。各麻醉组突触形 态改变无统计学意义。 结论麻醉及手术后早期可损害老年大鼠的认知功能，并引起海马胆碱乙酰 转移酶表达的减少，而海马突触的可塑性可能是术后认知功能障碍的结构基础。 关键词手术；认知；突触；胆碱乙酰转移酶 兰州大学硕：l：学位论文Effects 兰州大学硕：l：学位论文 Effects of splenectomy on cognition and synaptic plasticity and expressions of ChAT in the hippocampus of aged rats Abstract Objective To study the effects of surgery on cognition and quantitative analysis of ultrastructural changes in synapses of the rat hippocampal field CA3 and expressions of ChAT in the hippocampus of aged rats． e ’； Methods Total 56 aged rats were randomly divided into 3 groups：group C，A ，^ and O．In group A anesthesia was induced with ketamine 40mg／kg but did not perform the operation．In group O all aged rats were done splenectomy．Morris water maze test was used to observe cognition on daysl，3，7 after operation，respectively．According to the time of morris water maze test，group A and group O were divided into 3 subgroup，A1，A3，A7 and 01，03，07．End of the test，the expressions of ChAT in the hippocampus was measured，and synaptic structure was also measured in the polymorphic layer of the rat hippocampal field CA3． Results The latency period and swimming distance Were significantly longer in groupAl，01 and 03 than in other groups(all P0．05)．The latency period was longer in group01 than in groupAl俨O．05)．The expressions of ChAT in the hippocampus were decreased in group01 and 03 than other groups(allP0．01)．The width of synaptic cleft was longer