温度和pH对公子小丑鱼幼鱼消化酶活性的影响.pdfVIP

广东农业科学 2014 年第 3 期 131 温度和pH 对公子小丑鱼幼鱼消化酶活性的影响 张 弢 蔡生力 鞠晨曦 上海海洋大学水产与生命学院/农业部水产种质资源与养殖生态重点开 实验室袁上海 201306冤 摘要院研究了温度和 pH 对人工养殖下的公子小丑鱼 A mp hiprion oce llaris 冤幼鱼的胃尧肝脏尧肠道中蛋白酶尧 粉酶尧脂肪酶活性的影响袁并对各部位消化酶活性进行比较遥 结果表明袁在胃尧肝脏尧肠道中均可以检测出蛋白酶尧 粉酶尧脂肪酶的活性袁其中蛋白酶活性顺序为胃跃肠道跃肝脏袁 粉酶活性顺序为肠道跃肝脏跃胃袁脂肪酶活性顺序为肠 道跃肝脏跃胃遥 胃尧肝脏尧前肠尧中肠尧后肠蛋白酶的最适 pH 值分别为 2.2尧7.5尧7.0尧7.0 和 8.0 曰 粉酶肝脏的最适 pH 值 为 6.0 袁其余部位均为 7.0 曰脂肪酶肝脏的最适 pH 值为 7.0 袁其余部位均为 7.5遥 胃尧肝脏尧肠道的蛋白酶最适温度均为 40益曰胃 粉酶最适温度为 40益袁肝 粉酶最适温度为 35益袁肠道 粉酶最适温度为 30益曰胃尧肝脏尧肠道的脂肪酶的 最适温度均为 40益遥 关键词院公子小丑鱼曰温度曰pH 曰消化酶 中图分类号院S917 文献标识码院A 文章编号院1004-874X 2014冤03-0131-05 Effects of temperature and pH on activity of digestive enzymes in Amphiprion ocellaris j uvenile ZHANG Tao, CAI Sheng-li, JU Chen-xi Colle ge of Fis he rie s and Life Sc ie nce , Shanghai Oce an Unive rs ity/Key Laboratory of A q uatic G e ne tic Re sourc es and A q ua-c ultural Ec osys te m Ce rtifie d by the Minis try of A gric ulture , Shanghai 201306, China冤 Abstract: Effects of temperature and pH on activity of 3 kinds of digestive enzymes (protease, amylase and lipase) in stomach, liver and intestine of A mp hip rion oc e llaris j uvenile were studied and compared under artificial culture. The results showed that activity of protease, amylase and lipase all could be detected in stomach, liver and intestine, the order of protease activ ity was stomach intestinal liver, the order of amylase activity was intestinal liver stomach, the order of lipase activity was intestinal liver stomach. The optimum pH value