高氯酸超声萃取.pdf

/TobaccoScienceTechnology /TobaccoChemistry 2009 5 ( 262 ) - 1 2 1 1 1 , , 　, 　, 1.中国烟草总公司郑州烟草研究院, 郑州高新技术产业开发区枫杨街 2 号　450001 2.湖北中烟工业有限责 公司技术中心, 武汉市汉阳区十升路 22 号　4 30051 :超声萃取;高氯酸;淀粉;烟草;连续流动法 :为了快速测定烟草中的淀粉, 对比色法进行了 3 项改进:①用室温下超声萃取代替水浴加热萃取 除去干扰物;②用室温下超声萃取代替静置萃取提取烟草中的淀粉;③用连续流动法代替分光光度法测 定样品处理液中的淀粉,与比色法进行了对比,并采用改进法测定了 15 个国产烤烟烟叶和 15 个卷烟样 品中的淀粉含量。结果表明:①改进法的回收率为 97% ～100%,变异系数为 1.58%;②改进法的测定 结果较比色法偏高,连续流动法的测定结果略高于分光光度法;③相同年度不同产地的 K326 的 C3 F烟 叶中淀粉含量差异较大, 国产烤烟型卷烟的淀粉含量差异不大,进口混合型卷烟的淀粉含量比其低约 1 百分点。该法适用于烟草中淀粉的快速分析。 :TS411.1　:B　:1002-0861 (2009 )05 -0044 -04 DeterminationofStarchinTobaccobyUltrasonicExtractionandContinuousFloMethod WANGHONG-BO(1), XUYU-QIONG(2 ), WANGYING(1), ZHANGWEI(1), andLIUHUI-MIN(1) 1.ZhengzhouTobaccoResearchInstituteofCNTC, Zhengzhou45000 1, China 2.TechnologyCente,rHubeiBranchofChinaTobaccoIndustryCompany, Wuhan430051, China Keyord:sUltrasonicextraction;HClO;Starch;Tobacco;Continuousflomethod 4 Abstrac:tTodeterminestarchintobaccofastandaccuratel,ythecolormietricmethodasmiprovedasfolloing: 1)theinterferentialsubstancesinsample ereremovedbyultrasonicextractionatroomtemperatureinplaceof extractioninheatedater-bath;2 )thestarchintobaccoasextractedatroomtemperature ithultrasonicinstead ofstaticextraction;3 )thestarchintobaccoextractasdeterminedbyacontinuousfloautoanalyzerinplaceofa spectrophotomete.rThemiprovedmethod ascompared iththecolormietry.Starchcontentsin15 domesticflue- curedtobaccosamplesand15 commercialcigarettesampleseredetermined iththemiprovedmethod, andthe resultsshoedthattherecoveriesofstarcherefrom97% to100% iththecoefficientofvariationof1.58%.The starchcontentsdetermined iththemiprovedmethodandacontinuousfloautoanalyzerereslightlyhigherthan those iththecolormietryandspectrophotometr,yrespectively.ThestarchcontentsinC3 FofK326 fromdifferent groingareasofthesamecropyeardifferedobviousl,y hilethestarchcontents