慢性乙型肝炎相关肝癌发生、发展过程中的表观遗传学机制研究-遗传学专业毕业论文.docx

第二军医大学博士学位论文 第二军医大学博士学位论文 - - PAGE 4 - that specifically recognize the isoforms of Apo A-I may prove to be useful, in combination with other traditional markers, as a more efficient way to evaluate the prognosis of CHB. Second, our study increased understanding the relationships between clinical symptoms and molecular changes in HCC. Our findings suggest that the p38 MAPK pathway plays a crucial role in miR-17-5p-induced phosphorylation of heat shock protein 27 (HSP27) and, as a consequence, phosphorylated HSP27 enhances the migration of HCC cells. Our data highlight an important role of miR-17-5p in the proliferation and migration of HCC cells and support the potential application of miR-17-5p in HCC therapy. We also identified non-overlapping signatures of a small number of lncRNAs that are aberrantly expressed in human HCC compared with paired peritumoral tissues. Then we used real-time PCR to validate five lncRNAs whose expression was altered in HCC compared with paired peritumoral tissues. Using loss-of-function and gain-of-function approaches, we found that lncRNA-HEIH plays a key role in cell cycle regulation. We further demonstrated that lncRNA-HEIH bound to enhancer of zeste homolog 2 (EZH2) and that this interaction was required for the repression of EZH2 target genes. These results reveal insights into the molecular regulation mechanisms of HCC cell cycle regulation and lead us to propose that lncRNAs may serve as key regulatory hubs in cancer biology. The present study also provides new insights into the pathogenesis of hepatitis B virus infection, which might not be obtained by studying a specific individual molecule. In addition to a close connection between glutathione peroxidase 1and oxidative stress, our integrated approach highlighted the fact that fatty acid binding 5, Acyl-CoA binding protein and apo A-I could be the key points of lipid metabolism derangement in HBV-Tg mice. Our study also shed light on the physiological difference between HBV-T