黄芩素的提取分离、活性及其脂质体制备研究-无机化学专业毕业论文.docxVIP

黄芩索的提取分离、活性及其脂质体制备研究基自由基(DPPH·)和超氧自由基的清除作用以及-OH引发的胸腺核苷 黄芩索的提取分离、活性及其脂质体制备研究 基自由基(DPPH·)和超氧自由基的清除作用以及-OH引发的胸腺核苷 损伤的防护作用。结果表明，黄芩素对DPPH咱由基具有很强的清除 作用，且pH对清除率的影响很大，当pH=3时清除率最大，约为93％； 另外，黄芩素也能有效清除超氧阴离子自由基，并能起到一定的防护 胸腺核苷氧化损伤作用。 4．利用酶活力法对黄芩素体外抑制弹性蛋白酶活性进行了研究， 发现黄芩素对弹性蛋白酶具有很强的抑制作用，抑制率达92．98％。 研究提示：黄芩素可能具有改善肺组织纤维化的功能。 5．以包封率和载药量为评价指标，探讨了黄芩素脂质体的制备 方法；采用透射电镜和粒度分析仪考察了脂质体的形态学和粒径分布 状态。结果表明：逆相蒸发法制各的脂质体(平均粒径179nm，包封 率和载药量分别为92．57％和23．24％)质量优于薄膜超声法(平均粒 径257nrri，包封率和载药量分别为60．02％$N 7．70％)；黄芩素脂质体 在pH7．4磷酸盐缓冲液中的体外释药行为研究显示，15h内释药量为 57．13％，释放规律符合Higuchi方程释药模型。 关键词： 黄芩素：提取分离；稳定性：活性：脂质体 摘要ABSTRACT 摘要 ABSTRACT Baicalein 1S one of the effective ingredients of Chinese herbs Scutellaria baicalensis Georgi，it can antibiosis，antiviral，anticancer and other pharmacological actions．At the present time，extraction and separation of baicalein iS used to immersion method，which is time—consuming and low productive rate，and the studies are focus on its extraction and separation in our country，but extraction processing has never been reported yet．Consequently，it is necessary to search the effect factors of baicalein for extraction and separation． Free radicals are the general intermediates of biochemical reactions in organisms．When free radicals are produced excessively or removed extreme slowly,they will attack a lot of diseases．Therefore，screening natural antioxidant became foCHS at home and abroad．Because flavonoid has antioxidation，and baicalein is one of the flavonoids．Consequently， the scavenging effects of the baicalein were studied． Liposome has the character of covering fat—soluble drug and water —soluble drug．Fat—soluble drug present different characters and its solubi —lity of drug is increasing，when it was covered．Otherwise，liposome has targeting and releasing actions，and it can increase therapeutic effectiven —ess and reduce sideeffect．Because baicalein has low solubility in water and is oxidized easily．Preparation method and in vitro pharmacodynami —CS of baicalein liposome were researched to