抗狂犬病病毒单克隆抗体的制备以及初步应用研究-预防兽医学专业毕业论文.docxVIP

广膏上矿硕士毕业论文和E1—5具有良好的细胞中和活力。 广膏上矿硕士毕业论文 和E1—5具有良好的细胞中和活力。 关键词狂犬病病毒单克隆抗体制备应用 分泌抗狂犬病病毒单克隆抗体杂交瘤细胞株的建立以及初步应用．冯励PREPARATION 分泌抗狂犬病病毒单克隆抗体杂交瘤细胞株的建立以及初步应用．冯励 PREPARATION AND PRIMARY APPLICATION OF MONOCLONAL ANTIBoDY AGAINST RABIES VIRUS ABSTRACT Rabies is an age—old infectious disease of the central nervous system of major importance to human and veterinary medicine．It causes a fatal encephalomyelitis for which there is no treatment once the clinical symptoms have appeared．Vaccination is the only way to combat the infection．Nowadays the pathogenic mechanisms have been the SUbject of many studies，but are still not well understood。We need to know the pathogenic gene in order to root out the rabies． In this study，Rabies virus cell adapted strain ERA，grown in monolayer cultures of BHK一21 cells and purified by centrifngation， Zn(AC)z precipitation and subsequently 10～55％(w／v)sucrose density grasient·The purification have rabies virus of highly titer by RT—PCR． BALB／c mouse were immunized with the purified RV,mouse spleen cell were fused with myelomas SP2／0．Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA，positive colonies were cloned three times by limited dilution，five cell line while secrete McAbs against rabies virus were obtained．There five McAbs designated as E1-1，E1-2，E1．4，E1-5 and E1—6． 3 广母上∥硕士毕业论文The 广母上∥硕士毕业论文 The hybridomas were inoculated in the mice bellies to produce McAbs and the ascites McAbs titer had been measured between 1：2 000 to 1：8 000． All McAbs could react with four rabies virus strains(119，BM，LA，F一牛) collected from different county in Guangxi，indicating that five McAbs are anti-rabies virus．The MeAbs did not cross react with PRRSV、HCV、PCV and PRV,demonstrated good specificity．Three of five MeAbs(El一1，E1—2， E1—5)have highly cellular neutralizing abilities． KEY WORDS rabies virus；monoclonal antibody；preparation；primary application 4 分泌抗狂犬病病毒单克隆抗体杂交窟细胞株的建立以及初步应用．冯励前言 分泌抗狂犬病病毒单克隆抗体杂交窟细胞株的建立以及初步应用．冯励 前言 狂犬病是由狂犬病病毒