罗氏沼虾多倍体育种的研究-动物学专业毕业论文.docx

硕士学侄论文、f、‘f 硕士学侄论文 、f、‘f rR’c!1 7 T气I、 热休克诱导罗氏沼虾四倍体的最好条件是：在受精后210分钟时，用40 ℃水温处理胚胎1．5分钟，在这种条件下，最高可以得到35．86％的四倍体率。 鼢聊 4细胞松弛素B诱导罗氏沼虾四倍体 细胞松弛素B诱导罗氏沼虾四倍体的最好条件是：在受精后230分钟，用 1．Omg／L的C．B处理 10分钟，此时最高的四倍体诱导率为33．78％。 确 关键词罗氏沼虾四倍体诱导 硕士肇位论文’、：¨nI；’S 硕士肇位论文 ’、：¨nI；’S llli、- Abstract The giant fieshwater prawn(^4ac，．06，‘口c^f“，玎，．Ds已珂6e，苫ff)is a kind of prawn with great economic Value and is cultured Very abroad．This fkshwater prawn has been popular in Chinese Mainland due to its dejicious taste and abundant nutrition since it was introduced here．Now the prawn has bcen one of me most jmportant aquatic p『oducts in the矗eShwater cultivation．HoweVer， since the】ong—ternl anmcially intensiVe aquaculture，that is the only way used forthis pmwn now in Chinese Mainland，is Very easy to cause the problems such as being mature in advaIlce， reducing Of血e body size 柚d the weak resistance to adverse circumstance， so we researched On n坨polyploid induction of this prawn by c”ological techniques so as to s01Ve these problems．Generally the polyploids caJl grow flast and get a big size than the dipIoid， and they have stronger resistance to disadvafltageous environment．Furthenllore its taste is also nice．Here， r firstly investigated on the time of lhe extnlsion of the fifst and the second polar bodies and of the nrst cleaVage．Then I studied on the chromosome number of the wikl type embryos of this pra啪．On these basis， I successmlly induced the tetraploidy embryos of the giant freshwater prawn by heat-shock a11d c”ochalasin B(C．B) treatInent． 1．The inVestigation of the extrusion of the nrst and the second polar bodies and 0fthe疗璐t cleavage The tjme of the ex咖siDn of the first a11d the second polar bodies and of the nrst cleavage were investigated by paramn sections．We observed that the nrst pol盯body had been released when me ooc”e matured physiologically．By 55 min after the lnseminatlon，me secorId p01ar body w丛托le懿ed．nlc first cIeava2e iii 硕士竿位论文3蓟 硕士竿位论文 3蓟 、1、叫i R’K|1 7 r‘’＼ occurred印pmximately 210-23