美洲大蠊生物学观察及其提取物抗胃溃疡作用研究-中西结合基础专业毕业论文.docxVIP

摘 摘 要 为了给药用昆虫美洲大蠊的大量繁殖提供重要的科学依据和详 细的实验观察资料，同时也为进一步观察美洲大蠊提取物对不同种类 胃溃疡的保护作用。本研究全面系统的观察了美洲大蠊在人工饲养条 件下的室内繁殖、发育及其生物学特性和行为学特点，并对其提取物 进行了相应的药效学动物实验。 首先，在实验室自然温度下，我们观察了美洲大蠊的生殖产卵、 孵化、若虫生长、蜕皮、羽化等发育的全过程，以及各发育阶段的食 性行为特点，对每鞘含卵数、卵孵率、鞘孵率等生物学特征参数进行 分析和统计。其次，以美洲大蠊提取物作为试药，观察不同药物剂量 对冷水致小鼠应激型胃溃疡、小鼠醋酸烧灼型胃溃疡，及小鼠无水乙 醇致胃粘膜损伤型胃溃疡的保护作用。 通过生物学观察，我们发现美洲大蠊的产卵习性和食性受温度的 影响较大。动物试验结果也证实了美洲大蠊提取物对小鼠应激胃溃疡 型，及小鼠无水乙醇胃粘膜损伤型有较好的治疗效果，对小鼠醋酸烧 灼胃溃疡型则效果不太明显。 通过以上一系列观察与实验，我们认为美洲大蠊在饲养时应注意 控制好温度，并认为其人工提取物对于胃溃疡治疗的确具有进一步研 究开发的潜在价值。 关键词：美洲大蠊；生殖；生物学；发育；胃溃疡 AbstractIn Abstract In order to provide the experimental dam for artificial feeding and reproduction of medicinal insect Pa in large-scale，and observe The anti—gastrelcosis effects of water extracts from Periplaneta Americana in different dosage．Comprehensive and systematic investigation on domestic reproduction，development，biological characteristics and ethological features of Pa and pharmacod)rnamics animal experiment were carried out． at the natural temperature，the biological characteristics including reproduction of adults，laying eggs，hatching，moulting，emergence and the ethological features including negative phototaxis，aggregation，food habit at different developmental stages were observed。Biological parameters such as lengths of oothcae，distribution of oothcae containing eggs，eggs per oothcae，duration for eggs，hatching rate for eggs and oothcae，emergence rate ofmale and female adults，and reproductive period of female were determined。Inhibitive effects of water extracts were investigated in gastrelcosis induced by alarm reaction to cold water, gastrelcosis induced by acetic acid，gastrelcosis induced by dehydrated alcohoL we found that temperature effected laying eggs habit and food habit，greatly．and The resuks of animal experiment showed that the higher dosage Pa extracts played significant roles in anti-gastrelcosis induced by alarm reaction to cold water,anti—gastrelcosis induced by dehydrated alcohol，and there was obviously statistical difference，as compa